Exercise Prevents Glucocorticoid-Induced Myocardial 4-Hydroxynonenal Production

• Published in: Cardiovascular drugs and therapy 2023-08
• Main Authors: Hayta, Umit, Akin, Senay, Gungor, Irem, Colluoglu, Inci Tugce, Guray, Umit, Akin, Yesim, Demirel, Haydar A.
• Format: Article
• Language: English
• ISSN: 0920-3206; 1573-7241
• DOI: 10.1007/s10557-023-07506-4

PURPOSELong-term administration of glucocorticoids (GCs) increases myocardial oxidative stress. 4-Hydroxynonenal (4-HNE) protein adducts, a marker of oxidative damage, have been associated with several cardiovascular diseases, including atherosclerosis, cardiac hypertrophy, cardiomyopathy, and ischemia-reperfusion injury. Exercise training has been shown to have a protective effect on the heart by lowering the level of oxidative stress in cardiomyocytes. Therefore, we aimed to investigate the effect of long-term dexamethasone treatment and exercise training on myocardial 4-HNE levels. METHODSTwenty-four female Wistar albino rats were assigned to sedentary control-saline treated (C, n = 8), sedentary-dexamethasone treated (D, n = 8), and exercise training-dexamethasone treated (DE, n = 8) groups. Daily dexamethasone was injected for 28 days at a 1 mg kg-1 dose, while C animals were injected with the same volume of saline subcutaneously. DE animals underwent an exercise training protocol of 60 min/day, 5 days a week, at 25 m/min-1 (0% grade) for 28 days. Left ventricular 4-HNE, Hsp72 levels, and pHsp25/Hsp25 ratio were determined by Western blot. RESULTSThe administration of dexamethasone led to a significant elevation in 4-HNE levels in the myocardium of adult rats (p  0.05; DE vs. C). Exercise training induced a threefold increase in myocardial Hsp72 expression (p