Inflammasomes NLRP3 and AIM2 in peri‐implantitis: A cross‐sectional study

• Published in: Clinical oral implants research 2023-12, Vol.34 (12), p.1342-1353
• Main Authors: Galindo‐Moreno, Pablo, Montalvo‐Acosta, Saray, Martín‐Morales, Natividad, Carrillo‐Gálvez, Ana Belén, González‐Rey, Elena, O'Valle, Francisco, Padial‐Molina, Miguel
• Format: Article
• Language: English
• Subjects: AIM2; Biofilms; Biomarkers; Biopsy; Caspase 1 - metabolism; Caspase-1; Cross-Sectional Studies; DNA-Binding Proteins - metabolism; Gene expression; Humans; inflammasome; Inflammasomes; Inflammasomes - metabolism; Inflammation; Interleukin-1beta - analysis; Leukocytes; Lymphocytes; Lymphocytes B; Microenvironments; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; NLRP3; Peri-Implantitis; Periodontium; peri‐implant disease; Plasma cells; Tissues
• ISSN: 0905-7161; 1600-0501
• DOI: 10.1111/clr.14174

Background Inflammasome components NLRP3 and AIM2 contribute to inflammation development by the activation of caspase‐1 and IL‐1β. They have not been yet evaluated in samples from patients with active peri‐implantitis. Thus, the aim of the present study is to analyze the expression of inflammasomes NLRP3 and AIM2 and subsequent caspase 1 and IL‐1β assessing the microenvironment of leukocyte subsets in samples from patients with active peri‐implantitis. Methods Biopsies were collected from 33 implants in 21 patients being treated for peri‐implantitis. Biopsies from gingival tissues from 15 patients with healthy periodontium were also collected for control. These tissues were evaluated through conventional histological stainings. Then, immunohistochemical detection was performed to analyze NLRP3, AIM2, caspase‐1, and IL‐1β and markers of different leukocyte subsets. PCR for inflammasomes and related genes was also done. Results This manuscript reveals a high immunohistochemical and mRNA expression of NLRP3 and AIM2 inflammasomes, caspase‐1, and IL‐1β in biopsies collected from human peri‐implantitis. The expression of the tested markers was significantly correlated with the increase in inflammatory infiltrate, probing depth, presence of biofilm, and bleeding on probing. In these peri‐implantitis lesions, the area of biopsy tissue occupied by inflammatory infiltrate was intense while the area occupied by collagen was significantly lower. In comparison with periodontal healthy tissues, the inflammatory infiltrate was statistically significantly higher in the peri‐implantitis biopsies and was mainly composed of plasma cells, followed by T and B lymphocytes. Conclusion In human peri‐implantitis, chronic inflammation can be explained in part by the action of IL‐1β/caspase 1 induced through NLRP3 and AIM2 inflammasome activation.