LncRNA RPLP0P2 Promotes Colorectal Cancer Proliferation and Invasion via the miR-129-5p/Zinc Finger and BTB Domain-Containing 20 Axis

• Published in: Biochemical genetics 2024-06, Vol.62 (3), p.1556-1576
• Main Authors: Yuan, Hang, Yu, Peng, Wan, Zi-Ang, Chen, Bing-Chen, Tu, Shi-Liang
• Format: Article
• Language: English
• Subjects: Animals; Anticancer properties; Assaying; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cancer; Cell Line, Tumor; Cell migration; Cell Proliferation; Cell viability; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; E-cadherin; Female; Gene Expression Regulation, Neoplastic; Human Genetics; Humans; Immunofluorescence; Male; Medical Microbiology; Mice; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; Molecular modelling; N-Cadherin; Neoplasm Invasiveness; Non-coding RNA; Original Article; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Tumors; Vimentin; Wound healing; Xenotransplantation; Zinc; Zinc finger proteins; Zinc Fingers; Zoology
• ISSN: 0006-2928; 1573-4927; 1573-4927
• DOI: 10.1007/s10528-023-10478-7

We previously reported that long non-coding RNA (lncRNA) RPLP0P2 is involved in the progression of colorectal cancer (CRC); however, its molecular mechanisms in CRC remain unclear. In this study, we observed that RPLP0P2 was upregulated in CRC tissues and cell lines. Cell viability was measured using the MTT and colony formation assays. Migration and invasion capabilities were monitored by wound healing, transwell, and immunofluorescence assays. The results showed that RPLP0P2 downregulation inhibited cell viability, migration, and invasion capabilities of CRC cells, accompanied by decreased PCNA, N-cadherin, and Vimentin, and increased E-cadherin expression. Using the DIANA online database, miR-129-5p was identified as a downstream target of RPLP0P2. In fact, RPLP0P2 colocalized with miR-129-5p, acting as a miR-129-5p sponge. MiR-129-5p-inhibition almost abrogated the anti-tumor effects induced by RPLP0P2 inhibition in CRC cells. Zinc finger and BTB domain-containing 20 (ZBTB20) was identified as a potential downstream target of miR-129-5p in CRC cells. ZBTB20 overexpression prevented miR-129-5p mimic-mediated anti-tumor effects in CRC cells. A tumor xenograft assay was performed to monitor the role of RPLP0P2 in tumor growth. Of note, in tumor-bearing mice, RPLP0P2-silencing inhibited tumor growth, followed by increased miR-129-5p and decreased ZBTB20 expression. Our results suggest that lncRNA RPLP0P2 functions as an oncogene that promotes CRC cell proliferation and invasion via regulating the miR-129-5p/ZBTB20 axis, thus, it may serve as a candidate target for CRC interventional therapies.