Clinical implications of imprecise sampling time for 10‐ and 30‐min thyrotropin‐releasing hormone stimulation tests in horses

Background The thyrotropin‐releasing hormone (TRH) stimulation test is used to diagnose pituitary pars intermedia dysfunction (PPID) using 10‐ or 30‐min protocols. Imprecise sampling time for the 10‐min protocol can lead to misdiagnoses. Objectives To determine the effect of imprecise sampling time...

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Bibliographic Details
Published in:Equine veterinary journal 2024-03, Vol.56 (2), p.291-298
Main Authors: Vorster, Dante M., Wang, Wenqing, Kemp, Kate L., Bamford, Nicholas J., Bertin, François‐René
Format: Article
Language:English
Subjects:
adrenocorticotropic hormone
diagnostic test
endocrine
horse
Horses
laminitis
pituitary pars intermedia dysfunction
Protocol
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Summary:Background The thyrotropin‐releasing hormone (TRH) stimulation test is used to diagnose pituitary pars intermedia dysfunction (PPID) using 10‐ or 30‐min protocols. Imprecise sampling time for the 10‐min protocol can lead to misdiagnoses. Objectives To determine the effect of imprecise sampling time for the 30‐min protocol of the TRH stimulation test. Study design In vivo experiment. Methods Plasma immunoreactive adrenocorticotropin (ACTH) concentrations were measured 9, 10, 11, 29, 30 and 31 min after intravenous administration of 1 mg of TRH in 15 control and 12 PPID horses. Differences in ACTH concentrations between sampling times, variability in ACTH concentrations between protocols, and diagnostic classification of PPID were assessed using Friedman's test, Bland–Altman plots, and Fisher's exact test, respectively, with 95% confidence intervals reported and significance set at p 
ISSN:0425-1644
2042-3306
DOI:10.1111/evj.13991