Response surface optimisation of lipase-catalysed esterification of glycerol and\+i\n\-i\-3 polyunsaturated fatty acids from salmon oil

Enzymatic synthesis of acylglycerols from glycerol and n-3 fatty acid concentrate, prepared from salmon oil, was optimised by response surface methodology (RSM). Salmon oil was hydrolysed using a stereospecific sn-1, sn-3 lipase from Aspergillus oryzae and filtered on hydrophobic membrane. Acylglyce...

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Bibliographic Details
Published in:Process biochemistry (1991) 2005-01, Vol.40 (1), p.273-279.
Main Authors: Linder, M, Kochanowski, N, Fanni, J, Parmentier, M
Format: Article
Language:English
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Summary:Enzymatic synthesis of acylglycerols from glycerol and n-3 fatty acid concentrate, prepared from salmon oil, was optimised by response surface methodology (RSM). Salmon oil was hydrolysed using a stereospecific sn-1, sn-3 lipase from Aspergillus oryzae and filtered on hydrophobic membrane. Acylglycerols synthesis was performed using Lipozyme IM (Novo Nordisk) under different reaction conditions according to a Doehlert experimental design. Effects of temperature, water content, glycerol/fatty acid molar ratio, and enzyme/substrate ratio were studied. Pathway maximisation indicated that an esterification degree of 97% was obtained under the following conditions: glycerol/oil 1.2, temperature 46DGC, molecular sieves 3.5 g per 100 ml and enzyme/substrate 8.1.
ISSN:1359-5113
DOI:10.1016/j.procbio.2004.01.014