Discovery of cisplatin-binding proteins by competitive cysteinome profiling

Cisplatin is a widely used cancer metallodrug that induces cytotoxicity by targeting DNA and chelating cysteines in proteins. Here we applied a competitive activity-based protein profiling strategy to identify cisplatin-binding cysteines in cancer proteomes. A novel cisplatin target, MetAP1, was ide...

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Bibliographic Details
Published in:RSC chemical biology 2023-08, Vol.4 (9), p.67-674
Main Authors: Wang, Xianghe, Zhang, Yihai, Wang, Chu
Format: Article
Language:English
Subjects:
Chemistry
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Summary:Cisplatin is a widely used cancer metallodrug that induces cytotoxicity by targeting DNA and chelating cysteines in proteins. Here we applied a competitive activity-based protein profiling strategy to identify cisplatin-binding cysteines in cancer proteomes. A novel cisplatin target, MetAP1, was identified and functionally validated to contribute to cisplatin's cytotoxicity. A competitive activity-based protein profiling strategy was developed to identify cisplatin-binding cysteines in MCF-7 proteomes, among which cisplatin-binding sites in GLRX1, TXNDC17 and MetAP1 were biochemically verified.
ISSN:2633-0679
2633-0679
DOI:10.1039/d3cb00042g