Biodegradation of commercial textile reactive dye mixtures by industrial effluent adapted bacterial consortium VITPBC6: a potential technique for treating textile effluents

• Published in: Biodegradation (Dordrecht) 2024-04, Vol.35 (2), p.173-193
• Main Authors: Saha, Purbasha, Rao, Kokati Venkata Bhaskara
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Azo Compounds - metabolism; Bacteria - metabolism; Biodegradation; Biodegradation, Environmental; Biomedical and Life Sciences; CI Reactive Black 5; Coloring Agents - metabolism; Coloring Agents - toxicity; Consortia; Decoloring; Decolorization; Dichloroethane; Dyes; Effluents; Environmental perception; enzymes; Ethylene dichloride; fabrics; Geochemistry; Industrial effluents; Industrial wastes; Industrial wastewater; Kinetics; Life Sciences; Metabolites; Microbiology; Mixtures; Naphthalenesulfonates; Organometallic Compounds; Original Paper; Phytotoxicity; Reaction kinetics; Soil Science & Conservation; Sulfuric acid; Sulphuric acid; Terrestrial Pollution; Textile Industry; Textile industry wastes; Textile industry wastewaters; Textiles; Toxicity; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-023-10047-0

Textile industries release major fraction of dyestuffs in effluents leading to a major environmental concern. These effluents often contain more than one dyestuff, which complicates dye degradation. In this study ten reactive dyes (Reactive Yellow 145, Reactive Yellow 160, Reactive Orange 16, Reactive Orange 107, Reactive Red 195, Reactive Blue 21, Reactive Blue 198, Reactive Blue 221, Reactive Blue 250, and Reactive Black 5) that are used in textile industries were subjected to biodegradation by a bacterial consortium VITPBC6, formulated in our previous study. Consortium VITPBC6 caused single dye degradation of all the mentioned dyes except for Reactive Yellow 160. Further, VITPBC6 efficiently degraded a five-dye mixture (Reactive Red 195, Reactive Orange 16, Reactive Black 5, Reactive Blue 221, and Reactive Blue 250). Kinetic studies revealed that the five-dye mixture was decolorized by VITPBC6 following zero order reaction kinetic; V max and K m values of the enzyme catalyzed five-dye decolorization were 128.88 mg L −1  day −1 and 1003.226 mg L −1 respectively. VITPBC6 degraded the dye mixture into delta-3,4,5,6-Tetrachlorocyclohexene, sulfuric acid, 1,2-dichloroethane, and hydroxyphenoxyethylaminohydroxypropanol. Phytotoxicity, cytogenotoxicity, microtoxicity, and biotoxicity assays conducted with the biodegraded metabolites revealed that VITPBC6 lowered the toxicity of five-dye mixture significantly after biodegradation.