Role of some inflammasomes in rheumatoid arthritis patients in Egypt

Aim This study aims to demonstrate the role of some inflammasomes genes: NLRC4 (the NLR family, CARD domain-containing protein 4), NLRP1 (NLR family, pyrin domain-containing 1), ASC (Apoptosis-associated speck-like protein containing a CARD), and CASPASE-1 in the pathogenesis of Rheumatoid arthritis...

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Bibliographic Details
Published in:Molecular biology reports 2023-11, Vol.50 (11), p.8809-8815
Main Authors: Ibrahem, Reham, Raghip, Mervat A., Abdelwahed, Mamdouh M., Amin, Noha S., Abualfadl, Esam M., Waly, Nancy G. F. M.
Format: Article
Language:English
Subjects:
Adapter proteins
Animal Anatomy
Animal Biochemistry
Apoptosis
Arthritis, Rheumatoid - genetics
Autoimmune diseases
Biomedical and Life Sciences
blood serum
CARD Signaling Adaptor Proteins - genetics
CARD Signaling Adaptor Proteins - metabolism
Caspase 1 - genetics
Caspase 1 - metabolism
Caspase-1
Cytokines
Data analysis
Disease
Egypt
Enzymes
family
Genes
Glyceraldehyde-3-phosphate dehydrogenase
Histology
Humans
Illnesses
Immunology
Inflammasomes
Inflammasomes - genetics
Inflammasomes - metabolism
Inflammation
interleukin-18
Interleukin-1beta - metabolism
Life Sciences
Males
Molecular biology
Morphology
NLR Family, Pyrin Domain-Containing 3 Protein - metabolism
Original Article
Pathogenesis
Pathology
Pharmacy
Pyrin protein
quantitative polymerase chain reaction
Rheumatoid arthritis
Rheumatology
therapeutics
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Summary:Aim This study aims to demonstrate the role of some inflammasomes genes: NLRC4 (the NLR family, CARD domain-containing protein 4), NLRP1 (NLR family, pyrin domain-containing 1), ASC (Apoptosis-associated speck-like protein containing a CARD), and CASPASE-1 in the pathogenesis of Rheumatoid arthritis (RA) in Egyptian population. Main methods The expression level of NLRC4, NLRP1, ASC, and CASPASE-1 within PBMCs isolated from all RA subjects by quantitative real-time PCR. GAPDH gene was used as a reference gene. Measurement of serum level of IL-1β and IL-18 was performed using ELISA. Key findings Results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease. Significance Understanding the role of inflammasomes in RA pathogenesis helps in finding promising therapy for the treatment and management of this disease.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-023-08738-1