Nutrient condition modulates the antibiotic tolerance of Pseudomonas aeruginosa

• Published in: The Science of the total environment 2023-12, Vol.904, p.166749-166749, Article 166749
• Main Authors: Liu, Huizhong, Wang, Yingsi, Zhang, Zhiqing, Qi, Hong, Zhang, Yu, Li, Wenru, Shi, Qingshan, Xie, Xiaobao
• Format: Article
• Language: English
• Subjects: Antibiotic tolerance; Nutrient; Resistance genes; Survival strategy; Transcriptome
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.166749

The variation in nutrient content across diverse environments has a significant impact on the survival and metabolism of microorganisms. In this study, we examined the influence of nutrients on the antibiotic tolerance of the PAO1 strain of Pseudomonas aeruginosa. Our findings indicate that under nutrient-rich conditions, this strain exhibited relatively high tolerance to ceftazidime, chloramphenicol, and tetracycline, but not aminoglycosides and fluoroquinolones. Transcriptome analysis revealed that genes associated with antibiotic tolerance were expressed more efficiently in nutrient-rich media, including ribosomal protein genes and multidrug efflux pump genes, which conferred higher tetracycline tolerance to the strain. Furthermore, the genes responsible for translation, biosynthesis, and oxidative phosphorylation were suppressed when nutrients were limited, resulting in decreased metabolic activity and lower sensitivity to ciprofloxacin. Artificial interference with ATP synthesis utilizing arsenate confirmed that the curtailment of energy provision bolstered the observed tolerance to ciprofloxacin. In general, our results indicate that this strain of P. aeruginosa tends to activate its intrinsic resistance mechanisms in nutrient-rich environments, thereby enhancing resistance to certain antibiotics. Conversely, in nutrient-limited environments, the strain is more likely to enter a dormant state, which enables it to tolerate antibiotics to which it would otherwise be sensitive. These findings further suggest that antibiotics released in environments with varying eutrophication levels may have divergent effects on the development of bacterial antibiotic resistance. [Display omitted] •The antibiotic tolerance of P. aeruginosa is affected by nutrient conditions.•Eutrophic conditions are conducive to the expression of resistance genes.•High expression of efflux pumps and ribosome confers tetracycline tolerance.•Low metabolism caused by nutrient deficiency helps to tolerate ciprofloxacin.