Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF...

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Published in:Molecular and cellular endocrinology 2023-12, Vol.578, p.112049-112049, Article 112049
Main Authors: Krysta-Matter, A.E., Riepsamen, A.H., Lien, S., Wong, W.Y.T., Richani, D., Kilani, S., Harrison, C.A., Mallitt, K.A., Ledger, W.L., Robertson, D.M., Gilchrist, R.B.
Format: Article
Language:English
Subjects:
Bone morphogenetic protein 15
Cumulin
Cumulus cells
Growth differentiating factor 9
Oocyte
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Summary:Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p 
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2023.112049