FeNC nanozyme-based electrochemical immunoassay for sensitive detection of human epidermal growth factor receptor 2

The proof-of-concept of sensitive electrochemical immunoassay for the quantitative monitoring of human epidermal growth factor receptor 2 (HER2) is reported. The assay is carried out on iron nitrogen-doped carbon (FeNC) nanozyme-modified screen-printed carbon electrode using chronoamperometry. Intro...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2023-10, Vol.190 (10), p.378-378, Article 378
Main Authors: Qiu, Minghao, Ren, Yuqing, Huang, Lumin, Zhu, Xueying, Liang, Tikai, Li, Meijin, Tang, Dianping
Format: Article
Language:English
Subjects:
Analytical Chemistry
Carbon
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electric properties
Electrical measurement
Epidermal growth factor
Glucose oxidase
Growth factors
Hydrogen peroxide
Immunoassay
Immunosensors
Microengineering
Monoclonal antibodies
Nanochemistry
Nanotechnology
Original Paper
Oxidases
Receptors
Target detection
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Summary:The proof-of-concept of sensitive electrochemical immunoassay for the quantitative monitoring of human epidermal growth factor receptor 2 (HER2) is reported. The assay is carried out on iron nitrogen-doped carbon (FeNC) nanozyme-modified screen-printed carbon electrode using chronoamperometry. Introduction of target HER2 can induce the sandwiched immunoreaction between anti-HER2 monoclonal antibody-coated microplate and biotinylated anti-HER2 polyclonal antibody. Thereafter, streptavidin-glucose oxidase (GOx) conjugate is bonded to the detection antibody. Upon addition of glucose, 3,3′,5,5′-tetramethylbenzidine (TMB) is oxidized through the produced H 2 O 2 with the assistance of GOx and FeNC nanozyme. The oxidized TMB is determined via chronoamperometry. Experimental results revealed that electrochemical immunosensing system exhibited good amperometric response, and allowed the detection of target HER2 as low as 4.5 pg/mL. High specificity and long-term stability are acquired with FeNC nanozyme-based sensing strategy. Importantly, our system provides a new opportunity for protein diagnostics. Graphical Abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-023-05964-z