Facile extraction of berberine from different plants, separation, and identification by thin‐layer chromatography, high‐performance liquid chromatography, and biological evaluation against Leishmaniosis

The extraction of berberine was carried out from Berberis vulgaris , Berberis aquifolium , and Hydrastis canadensis plants using ethanol and water (70:30, v/v). The extracted berberine was characterized by ultraviolet‐visible and Fourier‐transform infrared spectroscopy. The purity of berberine was a...

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Bibliographic Details
Published in:Journal of separation science 2023-11, Vol.46 (21), p.e2300582-e2300582
Main Authors: Alam, Syed Dilshad, Beg, Mirza Adil, Bagadi, Muralidhararao, Locatelli, Marcello, ALOthman, Zeid A., Mustaqeem, Mohd, Ali, Imran
Format: Article
Language:English
Subjects:
Acetonitrile
Ammonium acetate
Analytical chemistry
Chromatography
Ethanol
Formic acid
Fourier transforms
High performance liquid chromatography
Separation
Thin layer chromatography
Zodiac
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Summary:The extraction of berberine was carried out from Berberis vulgaris , Berberis aquifolium , and Hydrastis canadensis plants using ethanol and water (70:30, v/v). The extracted berberine was characterized by ultraviolet‐visible and Fourier‐transform infrared spectroscopy. The purity of berberine was ascertained by thin‐layer chromatography using n ‐propanol‐formic acid‐water (95:1:4) and (90:1:9) solvents. h R f values were in the range of 44–49 with compact spots (diameter 0.2–0.4 cm). HPLC was carried out using ammonium acetate buffer and acetonitrile in gradient mode with Zodiac (4.6 × 150 mm, 3 μm) column. The flow rate was 1.0 mL/min and detection was at 220 nm. The values of separation and resolution factors of the standard and the extracted berberine were in the range of 1.13–1.16 and 1.40–1.71, respectively. A comparison has shown that both thin‐layer chromatography and high‐performance liquid chromatography (HPLC) methods found applications in different situations and requirements. The extracted berberine samples were used to treat Leishmaniosis and the results showed better activity of berberine in comparison to the standard drug Amphotericin B. Briefly, the reported research is a novel and may be used to extract berberine from plants, separation and identification of berberine by thin layer chromatography and HPLC and to treat Leishmaniosis.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202300582