Thermodynamic and mechanistic analysis of the functional properties of dengue virus NS3 helicase

The Dengue Virus (DENV) non-structural protein 3 (NS3) is a multi-functional protein critical in the viral life cycle. The DENV NS3 is comprised of a serine protease domain and a helicase domain. The helicase domain itself acts as a molecular motor, either translocating in a unidirectional manner al...

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Bibliographic Details
Published in:Biophysical reviews 2023-08, Vol.15 (4), p.591-600
Main Authors: Incicco, J. Jeremías, Cababie, Leila A., Sarto, Carolina, Adler, Natalia S., Amrein, Fernando, Mikkelsen, Evelyn, Arrar, Mehrnoosh, Kaufman, Sergio B.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biological Techniques
Biomedical and Life Sciences
Biophysics
Cell Biology
Dengue fever
DNA helicase
Double-stranded RNA
Life cycles
Life Sciences
Membrane Biology
Molecular motors
Nanotechnology
Nucleoside triphosphates
Proteins
Review
Serine proteinase
Thermodynamics
Unwinding
Viruses
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Summary:The Dengue Virus (DENV) non-structural protein 3 (NS3) is a multi-functional protein critical in the viral life cycle. The DENV NS3 is comprised of a serine protease domain and a helicase domain. The helicase domain itself acts as a molecular motor, either translocating in a unidirectional manner along single-stranded RNA or unwinding double-stranded RNA, processes fueled by the hydrolysis of nucleoside triphosphates. In this brief review, we summarize our contributions and ongoing efforts to uncover the thermodynamic and mechanistic functional properties of the DENV NS3 as an NTPase and helicase.
ISSN:1867-2450
1867-2469
DOI:10.1007/s12551-023-01101-5