Cerebellar Nuclei Receiving Orofacial Proprioceptive Signals through the Mossy Fiber Pathway from the Supratrigeminal Nucleus in Rats

• Published in: Cerebellum (London, England) England), 2024-10, Vol.23 (5), p.1795-1810
• Main Authors: Tsutsumi, Yumi, Morita, Yayoi, Sato, Fumihiko, Furuta, Takahiro, Uchino, Katsuro, Sohn, Jaerin, Haque, Tahsinul, Bae, Yong Chul, Niwa, Hitoshi, Tachibana, Yoshihisa, Yoshida, Atsushi
• Format: Article
• Language: English
• Subjects: Animals; Axons; Biomedical and Life Sciences; Biomedicine; Brain stem; Cerebellar Nuclei - physiology; Cerebellum; Executive function; Facial Muscles - innervation; Facial Muscles - physiology; Interpositus nucleus; Male; Motor task performance; Muscle spindles; Muscle Spindles - physiology; Muscles; Neural Pathways - physiology; Neurobiology; Neurology; Neurosciences; Presynapse; Proprioception; Proprioception - physiology; Rats; Rats, Wistar; Spinal cord; Vestibular system
• ISSN: 1473-4230; 1473-4222; 1473-4230
• DOI: 10.1007/s12311-023-01602-9

Proprioception from muscle spindles is necessary for motor function executed by the cerebellum. In particular, cerebellar nuclear neurons that receive proprioceptive signals and send projections to the lower brainstem or spinal cord play key roles in motor control. However, little is known about which cerebellar nuclear regions receive orofacial proprioception. Here, we investigated projections to the cerebellar nuclei from the supratrigeminal nucleus (Su5), which conveys the orofacial proprioception arising from jaw-closing muscle spindles (JCMSs). Injections of an anterograde tracer into the Su5 resulted in a large number of labeled axon terminals bilaterally in the dorsolateral hump (IntDL) of the cerebellar interposed nucleus (Int) and the dorsolateral protuberance (MedDL) of the cerebellar medial nucleus. In addition, a moderate number of axon terminals were ipsilaterally labeled in the vestibular group Y nucleus (group Y). We electrophysiologically detected JCMS proprioceptive signals in the IntDL and MedDL. Retrograde tracing analysis confirmed bilateral projections from the Su5 to the IntDL and MedDL. Furthermore, anterograde tracer injections into the external cuneate nucleus (ECu), which receives other proprioceptive input from forelimb/neck muscles, resulted in only a limited number of ipsilaterally labeled terminals, mainly in the dorsomedial crest of the Int and the group Y. Taken together, the Su5 and ECu axons almost separately terminated in the cerebellar nuclei (except for partial overlap in the group Y). These data suggest that orofacial proprioception is differently processed in the cerebellar circuits in comparison to other body-part proprioception, thus contributing to the executive function of orofacial motor control.