Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

Food safety problems have become one of the most important public health issues worldwide. Therefore, the development of rapid, effective and robust detection is of great importance. Amongst a range of methods, nucleic acid isothermal amplification (NAIA) plays a great role in food safety detection....

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Bibliographic Details
Published in:Critical reviews in food science and nutrition 2024-12, Vol.64 (32), p.12061-12082
Main Authors: Qiao, Jiali, Zhao, Zhiying, Li, Yaru, Lu, Minghui, Man, Shuli, Ye, Shengying, Zhang, Qiang, Ma, Long
Format: Article
Language:English
Subjects:
Adaptability
challenges and future perspectives
CRISPR
CRISPR-Cas Systems
CRISPR/Cas
detection limit
Food Contamination - analysis
Food industry
Food Microbiology - methods
Food safety
Food Safety - methods
food safety detection
Humans
Nucleic Acid Amplification Techniques - methods
nucleic acid detection
nucleic acid isothermal amplification
Nucleic acids
nutrition
Public health
Safety
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Summary:Food safety problems have become one of the most important public health issues worldwide. Therefore, the development of rapid, effective and robust detection is of great importance. Amongst a range of methods, nucleic acid isothermal amplification (NAIA) plays a great role in food safety detection. However, the widespread application remains limited due to a few shortcomings. CRISPR/Cas system has emerged as a powerful tool in nucleic acid detection, which could be readily integrated with NAIA to improve the detection sensitivity, specificity, adaptability versatility and dependability. However, currently there was a lack of a comprehensive summary regarding the integration of NAIA and CRISPR/Cas in the field of food safety detection. In this review, the recent advances in food safety detection based on CRISPR/Cas-integrated NAIA were comprehensively reviewed. To begin with, the development of NAIA was summarized. Then, the types and working principles of CRISPR/Cas were introduced. The applications of the integration of NAIA and CRISPR/Cas for food safety were mainly introduced and objectively discussed. Lastly, current challenges and future opportunities were proposed. In summary, this technology is expected to become an important approach for food safety detection, leading to a safer and more reliable food industry.
ISSN:1040-8398
1549-7852
1549-7852
DOI:10.1080/10408398.2023.2246558