Improving the Durability of Chitosan Films through Incorporation of Magnesium, Tungsten, and Graphene Oxides for Biomedical Applications

• Published in: Chemistry & biodiversity 2023-11, Vol.20 (11), p.e202301018-n/a
• Main Authors: Alaithan, Fatimah, Khalaf, Mai M., Gouda, Mohamed, Yousef, T. A., Kenawy, Sayed H., Abou‐Krisha, Mortaga M., Abou Taleb, Manal F., Shaaban, Saad, Alkars, Abdullah M., Abd El‐Lateef, Hany M.
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Bacteria; Bacterial diseases; biomedical applications; Biomedical materials; Cell viability; Chitosan; E coli; Electron microscopes; Fourier transforms; Graphene; Infrared analysis; Infrared spectroscopy; Magnesium; Magnesium oxide; Medical dressings; Membranes; Nanocomposites; Polymers; Scaffolds; Scanning electron microscopy; Superconductors (materials); thermal stability; Thermogravimetric analysis; Tungsten; Tungsten oxide; Tungsten oxides; WO3/MgO/GO@CS scaffold; wound dressings, nanocomposites; Wound healing
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.202301018

Bacterial infections that cause chronic wounds provide a challenge to healthcare worldwide because they frequently impede healing and cause a variety of problems. In this study, loaded with tungsten oxide (WO3), Magnesium oxide (MgO), and graphene oxide (GO) on chitosan (CS) membrane, an inexpensive polymer casting method was successfully prepared for wound healing applications. All fabricated composites were characterized by X‐ray powder diffraction (XRD), Fourier transforms infrared spectroscopy (FT‐IR), and thermogravimetric analysis (TGA). A scanning electron microscope (SEM) was used to study the synthesized film samples’ morphology as well as their microstructure. The formed WO3/MgO@CS shows a great enhancement in the UV/VIS analysis with a highly intense peak at 401 nm and a narrow band gap (3.69 eV) compared to pure CS. The enhanced electron‐hole pair separation rate is responsible for the WO3/MgO/GO@CS scaffold's antibacterial activity. Additionally, human lung cells were used to determine the average cell viability of nanocomposite scaffolds and reached 121 % of WO3/MgO/GO@CS nanocomposite, and the IC50 value was found to be 1654 μg/mL. The ability of the scaffold to inhibit the bacteria has been tested against both E. coli and S. aureus. The 4th sample showed an inhibition zone of 11.5±0.5 mm and 13.5±0.5 mm, respectively. These findings demonstrate the enormous potential for WO3/MgO/GO@CS membrane as wound dressings in the clinical management of bacterially infected wounds.