Effects of cyclic adenosine monophosphate modulating agents during oocyte pre-maturation and the role of melatonin on in vitro maturation of bovine cumulus-oocyte complexes

This study investigated the effects of cyclic adenosine monophosphate modulating during cumulus-oocyte complexes (COCs) pre-maturation and the role of melatonin on in vitro maturation (IVM) of bovine COCs. In experiment one, COCs were pre-matured for 8 h in control medium or with 3-isobutyl-1-methyl...

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Published in:Animal reproduction science 2023-10, Vol.257, p.107327-107327, Article 107327
Main Authors: Silva, Bianca R., Barrozo, Laryssa G., Nascimento, Danisvânia R., Costa, Francisco C., Azevedo, Venância A.N., Paulino, Laís R.F.M., Lopes, Everton P.F., Batista, Ana L.P.S., Aguiar, Francisco L.N., Peixoto, Christina A., Donato, Mariana A.M., Rodrigues, Ana P.R., Silva, José R.V.
Format: Article
Language:English
Subjects:
Antioxidant
in vitro pre-maturation
Meiotic resumption
Melatonin and cAMP
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Summary:This study investigated the effects of cyclic adenosine monophosphate modulating during cumulus-oocyte complexes (COCs) pre-maturation and the role of melatonin on in vitro maturation (IVM) of bovine COCs. In experiment one, COCs were pre-matured for 8 h in control medium or with 3-isobutyl-1-methylxanthine (IBMX) and forskolin, IBMX and C-type natriuretic peptide, c-type natriuretic peptide and forskolin or IBMX, forskolin and c-type natriuretic peptide. Then, meiotic progression was evaluated. In experiment two, COCs were pre-matured, followed by IVM in control medium alone or with 10−6, 10−7 or 10−8 M melatonin. After IVM, chromatin configuration, transzonal projections (TZPs), reactive oxygen species, mitochondrial distribution, ultrastructure and mRNA expression for antioxidant enzymes were evaluated. In experiment 1, COCs pre-matured with both C-type natriuretic peptide and forskolin or C-type natriuretic peptide, forskolin and IBMX had lower meiotic resumption rate when compared to control. Considering that IBMX had not an additional effect to potentiate inhibition of meiotic resumption, a combination of C-type natriuretic peptide and forskolin was chosen. In experiment 2, COCs matured with 10−8 M melatonin had greater rates of meiotic resumption when compared to the other treatments (P 
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2023.107327