Elicitation of E-waste (acrylonitrile-butadiene styrene) enriched soil bioremediation and detoxification using Priestia aryabhattai MGP1

• Published in: Environmental research 2023-12, Vol.238, p.117126-117126, Article 117126
• Main Authors: Chakraborty, Moumita, Sharma, Barkha, Ghosh, Ankita, Sah, Diksha, Rai, J.P.N.
• Format: Article
• Language: English
• Subjects: Acrylonitrile-butadiene styrene (ABS); Biodegradation; E-polymer; E-Waste; Enrichment technique; Phytotoxicity assay
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2023.117126

Given the rise in both usage and disposal of dangerous electronics, there is a catastrophic rise in assemblage of electronic waste (e-waste). E-waste including various plastic resins are among the most frequently discarded materials in electronic gadgets. In current digital era, managing e-waste has become universal concern. From the viewpoint of persisting lacuna of e-waste managing methods, the current study is designed to fabricate an eco-friendly e-waste treatment with native soil bacteria employing an enrichment culture method. In the presence of e-waste, indigenous soil microbes were stimulated to degrade e-waste. Microbial cultures were isolated using enrichment medium containing acrylonitrile-butadiene styrene (ABS) as the primary carbon source. Priestia aryabhattai MGP1 was found to be the most dominant e-polymer degrading bacterial isolate, as it was reported to degrade ABS plastic in disposed-off television casings. Furthermore, to increase degradation potential of MGP1, Response Surface Methodology (RSM) was adopted which resulted in optimized conditions (pH 7, shaking-speed 120 rpm, and temperature 30 °C), for maximum degradation (18.88%) after 2 months. The structural changes induced by microbial treatment were demonstrated by comparing the findings of Field emission scanning electron microscopy (FESEM) images and Fourier Transform Infrared (FTIR) spectra confirming the disappearance of ≡ C─H peaks along with C–H, C=C and C ≡N bond destabilization following degradation. Energy-dispersive X-ray (EDX) analyzers of the native and decomposed e-polymer samples revealed a considerable loss in elemental weight % of oxygen by 8.4% and silica by 0.5%. Magnesium, aluminium and chlorine which were previously present in the untreated sample, were also removed after treatment by the bacterial action. When seeds of Vigna radiata were screened using treated soil in the presence of both e-waste and the chosen potent bacterial strain, it was also discovered that there was reduced toxicity in terms of improved germination and growth metrics as a phytotoxicity criterion. [Display omitted] •E-waste degrading bacteria stimulated with ABS polymer using soil enrichment method.•Priestia aryabhattai MGP1 degraded e-polymer as sole carbon source.•BBD was adopted to optimize degradation process.•FTIR, FESEM, and EDX validated disintegration of ABS•Biodegradation reduced e-waste toxicity on Vigna radiata.