Peripheral T Cell Populations are Differentially Affected in Familial Mediterranean Fever, Chronic Granulomatous Disease, and Gout

• Published in: Journal of clinical immunology 2023-11, Vol.43 (8), p.2033-2048
• Main Authors: Al, Burcu, Bruno, Mariolina, Röring, Rutger J., Moorlag, Simone J. C. F. M., Suen, Tsz Kin, Klück, Viola, Liu, Ruiqi, Debisarun, Priya A., Gaal, Orsolya, Bhat, Jaydeep, Kabelitz, Dieter, van de Veerdonk, Frank L., Joosten, Leo A.B., Netea, Mihai G., Placek, Katarzyna
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; CD4 antigen; CD8 antigen; CD8-Positive T-Lymphocytes; Cell activation; Chronic granulomatous disease; Congenital defects; Cytokines; Cytotoxicity; Familial Mediterranean Fever; Flow cytometry; Gout; Granulomatous Disease, Chronic - diagnosis; Humans; Hyperreactivity; Immunology; Immunoregulation; Infectious Diseases; Inflammasomes; Inflammatory diseases; Internal Medicine; Lymphocytes; Lymphocytes T; Medical Microbiology; Memory cells; Metabolic pathways; Metabolic rate; Original Article; Pathology; Phenotypes; Production capacity; γ-Interferon
• ISSN: 0271-9142; 1573-2592
• DOI: 10.1007/s10875-023-01576-7

Both innate errors of immunity, such as familial Mediterranean fever (FMF) and chronic granulomatous disease (CGD), and the common inflammatory disease gout are characterized by episodes of sterile inflammatory attacks in the absence of an infection. While these disorders encompass distinct pathologies due to differentially affected metabolic pathways and inflammasome activation mechanisms, their common features are the excessive production of interleukin (IL)-1ß and innate immune cell hyperreactivity. On the other hand, the role of T cells and innate-like lymphocytes such as gamma delta (γδ) T cells in these pathologies is ill-defined. In order to widen our understanding of T cell involvement in CGD, FMF and gout pathology, we developed multicolour immunophenotyping panels for flow cytometry to characterize γδ T cells as well as CD4 and CD8 T cell populations in terms of their cytokine production, activation status, memory or naive phenotypes, exhaustion status, homing receptor expression, and cytotoxic activity. Our study is the first deep immunophenotyping analysis of T cell populations in CGD, FMF, and gout patients. We found that CGD affects the frequencies and activation status of T cells, while gout impairs the cytokine production capacity of Vδ2 T cells. FMF was characterized by decreased percentages of regulatory T cells in circulation and attenuated IFN-γ production capacity by Vδ2 T cells. Autoinflammatory syndromes and congenital defects of phagocyte differentially affect T cell compartments. Future studies are warranted to assess whether these phenotypical changes are relevant for disease pathology.