Dynamics of imprinted genes and their epigenetic mechanisms in castor bean seed with persistent endosperm

• Published in: The New phytologist 2023-12, Vol.240 (5), p.1868-1882
• Main Authors: Han, Bing, Li, Yelan, Wu, Di, Li, De-Zhu, Liu, Aizhong, Xu, Wei
• Format: Article
• Language: English
• Subjects: Alleles; Arabidopsis - genetics; Arabidopsis Proteins - metabolism; Beans; Demethylation; Deoxyribonucleic acid; DNA; DNA (Cytosine-5-)-Methyltransferases - genetics; DNA methylation; DNA Methylation - genetics; DNA methyltransferase; Endosperm; Endosperm - genetics; Endosperm - metabolism; Epigenesis, Genetic; Epigenetics; Flowering; Gene expression; Gene Expression Regulation, Plant; Gene silencing; Genes; Genomes; Genomic Imprinting; Germination; Histones; Imprinting; Plants (botany); Ricinus communis; Ricinus communis - genetics; Ricinus communis - metabolism; Seed germination; Seeds; Seeds - metabolism
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.19265

Genomic imprinting refers to parent-of-origin-dependent gene expression and primarily occurs in the endosperm of flowering plants, but its functions and epigenetic mechanisms remain to be elucidated in eudicots. Castor bean, a eudicot with large and persistent endosperm, provides an excellent system for studying the imprinting. Here, we identified 131 imprinted genes in developing endosperms and endosperm at seed germination phase of castor bean, involving into the endosperm development, accumulation of storage compounds and specially seed germination. Our results showed that the transcriptional repression of maternal allele of DNA METHYLTRANSFERASE 1 (MET1) may be required for maternal genome demethylation in the endosperm. DNA methylation analysis showed that only a small fraction of imprinted genes was associated with allele-specific DNA methylation, and most of them were closely associated with constitutively unmethylated regions (UMRs), suggesting a limited role for DNA methylation in controlling genomic imprinting. Instead, histone modifications can be asymmetrically deposited in maternal and paternal genomes in a DNA methylation-independent manner to control expression of most imprinted genes. These results expanded our understanding of the occurrence and biological functions of imprinted genes and showed the evolutionary flexibility of the imprinting machinery and mechanisms in plants.