Structure‐based biological investigations on ruthenium complexes containing 2,2′‐bipyridine ligands and their applications in photodynamic therapy as a potential photosensitizer

• Published in: Chemical biology & drug design 2023-12, Vol.102 (6), p.1506-1520
• Main Authors: Puttaswamy, Naveen Y., Mahanta, Pranami, Sarma, Pranjit, Medhi, Chitrani, Kaid, Sanaa Mohammed Abdu, Kullaiah, Byrappa, Basumatary, Debajani, Manjasetty, Babu A.
• Format: Article
• Language: English
• ISSN: 1747-0277; 1747-0285
• DOI: 10.1111/cbdd.14341

Ruthenium complexes have been investigated for various biological applications by virtue of their radical scavenging, DNA binding, receptor binding, and cytotoxic abilities; especially the possible potential application of these complexes in photodynamic therapy (PDT). This study focuses on the synthesis, structural characterization and biological application (pertaining to its cytotoxicity and radical generation) of ruthenium complexed with salicylaldehyde fumaryl‐dihydrazone (slfhH 4 ), salicylaldehyde glutaryl‐di‐hydrazone (slfgH 4 ) and 2,2′‐bipyridine (bpy). During the synthesis, the anticipated complex was precipitated out but as serendipity, Ruthenium(II) tris (2,2′‐bipyridyl) monochloride nonahydrate {[Ru(bpy) 3 ] 2+ .Cl.9H 2 O} (RBMN) and Ruthenium(II) tris (2,2′‐bipyridyl) monochloride septahydrate {[Ru(bpy) 3 ] 2+ .Cl.7H 2 O}(RBMS) were crystallized from the filtrate. The crystal structure of complexes RBMN and RBMS were determined by a single‐crystal X‐ray diffraction methods and it showed that chlorine anion lies at the crystallographic axis and forms a halogen hydrogen‐bonded organic framework (XHOF) to provide the stability. In comparison with similar structures in Cambridge Crystallographic Data Center (CCDC) revealed that the nature of the XHOF framework and the layered packing are conserved. The compounds showed excellent cytotoxic ability (against L6 cells) and the nitro blue tetrazolium (NBT) assay upon irradiation to light revealed its ability to produce reactive oxygen species (ROS). The presence of partially occupied water molecules in the layered organization within the crystal packing mimics the release of ROS resulting in cytotoxicity. The structural results together with the biological data make these complexes interesting candidates for potential photosensitizers for PDT applications.