High-cell-density cultivation of Vibrio natriegens in a low-chloride chemically defined medium

Vibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has high potential as a production strain for biotechnological production processes or other applications in biotechnology. Culture media for V. natriegens typically...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2023-12, Vol.107 (23), p.7043-7054
Main Authors: Biener, Richard, Horn, Thomas, Komitakis, Alexander, Schendel, Ines, König, Leon, Hauenstein, Anna, Ludl, Alina, Speidel, Andrea, Schmid, Svenja, Weißer, Julian, Broßmann, Max, Kern, Sofia, Kronmüller, Max, Vierkorn, Sonja, Suckow, Lennart, Braun, Arthur
Format: Article
Language:English
Subjects:
Ammonium
Analysis
Bacteria
Batch culture
Biomass
Biomedical and Life Sciences
Bioreactors
Biotechnological Products and Process Engineering
Biotechnology
Cell culture
Cell density
Chloride
Citrates
Corrosion
Cultivation
Culture media
Density
Fed batch
Identification and classification
Life Sciences
Magnesium
Magnesium phosphate
Metal surfaces
Microbial Genetics and Genomics
Microbiology
Phosphates
Precipitates
Proteobacteria
Sodium
Sodium chloride
Sodium citrate
Sodium salts
Sulfates
Vibrio natriegens
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Summary:Vibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has high potential as a production strain for biotechnological production processes or other applications in biotechnology. Culture media for V. natriegens typically contain high sodium chloride concentrations. The corresponding high chloride concentrations can lead to corrosion processes on metal surfaces in bioreactors. Here we report the development of a low-chloride chemically defined medium for V. natriegens . Sodium chloride was completely replaced by the sodium salts disodium hydrogen phosphate, disodium sulfate, and sodium citrate, while keeping the total concentration of sodium ions constant. The use of citrate prevents the occurrence of precipitates, especially of ammonium magnesium phosphate. With this defined medium, high-cell-density fed-batch cultivations in laboratory-scale bioreactors using exponential feeding yielded biomass concentrations of more than 60 g L −1 . Key points A defined medium for V. natriegens that only contains traces of chloride was developed Corrosion processes on metal surfaces in industrial bioreactors can thus be prevented High yields of biomass can be achieved in fed-batch cultivation with this medium
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-023-12799-4