Combined effects of diesel and air exposure on oxidative stress parameters of mussels Perna perna (Mytilidae, Bivalvia)

• Published in: Marine pollution bulletin 2023-10, Vol.195, p.115559-115559, Article 115559
• Main Authors: Nogueira, Lílian, Garcia, Danielly, Cortez, Fernando Sanzi, Pereira, Camilo Dias Seabra, de Almeida, Eduardo Alves
• Format: Article
• Language: English
• Subjects: Antioxidant enzymes; Bivalves; Diesel effects; Hypoxia; Lipid peroxidation; Pollutants
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2023.115559

This study aimed to assess the combined effect of hypoxia and exposure to diesel on biochemical parameters of Perna perna mussels. Mussels previously kept for 48 h in clean seawater were submitted to hypoxia for 24 h followed by reoxygenation in clean seawater for 48 h. The same procedure was done but using seawater containing 0.01 mL/L of diesel, before and after hypoxia. Antioxidant enzymes as well as levels of glutathione and lipid peroxidation were measured in gills and digestive glands. The neutral red retention time assay was also evaluated in hemocytes. Results showed that cycles of air exposure and reoxygenation caused oxidative stress and antioxidant modulation in both the gills and digestive glands. The presence of diesel in water triggered additional modulation of antioxidants under hypoxia and reoxygenation stress, apparently enhancing the capacity of mussels to avoid lipid peroxidation. [Display omitted] •Hypoxia and reoxygenation caused oxidative stress and antioxidant modulation in Perna perna mussels.•Diesel in water affects mussel's responses to cycles of air exposure and reoxygenation.•GSH levels seemed to correlate with membrane lysosomal integrity in hemocytes.