The vacuolar Ca2+ transporter CATION EXCHANGER 2 regulates cytosolic calcium homeostasis, hypoxic signaling, and response to flooding in Arabidopsis thaliana

Flooding represents a major threat to global agricultural productivity and food security, but plants are capable of deploying a suite of adaptive responses that can lead to short‐ or longer‐term survival to this stress. One cellular pathway thought to help coordinate these responses is via flooding‐...

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Bibliographic Details
Published in:The New phytologist 2023-12, Vol.240 (5), p.1830-1847
Main Authors: Bakshi, Arkadipta, Won‐Gyu Choi, Su‐Hwa Kim, Gilroy, Simon
Format: Article
Language:English
Subjects:
Agricultural production
Ca2+-transporting ATPase
Calcium
Calcium homeostasis
Calcium ions
Calcium signalling
Calcium transport
Cations
Flooding
Food plants
Food security
Gene expression
Genes
Homeostasis
Hypoxia
Kinetics
Mutants
Signalling systems
Survival
Transcriptomics
Waterlogged ground
Waterlogging
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Summary:Flooding represents a major threat to global agricultural productivity and food security, but plants are capable of deploying a suite of adaptive responses that can lead to short‐ or longer‐term survival to this stress. One cellular pathway thought to help coordinate these responses is via flooding‐triggered Ca2+ signaling.We have mined publicly available transcriptomic data from Arabidopsis subjected to flooding or low oxygen stress to identify rapidly upregulated, Ca2+‐related transcripts. We then focused on transporters likely to modulate Ca2+ signals. Candidates emerging from this analysis included AUTOINHIBITED Ca2+ ATPASE 1 and CATION EXCHANGER 2. We therefore assayed mutants in these genes for flooding sensitivity at levels from growth to patterns of gene expression and the kinetics of flooding‐related Ca2+ changes.Knockout mutants in CAX2 especially showed enhanced survival to soil waterlogging coupled with suppressed induction of many marker genes for hypoxic response and constitutive activation of others. CAX2 mutants also generated larger and more sustained Ca2+ signals in response to both flooding and hypoxic challenges.CAX2 is a Ca2+ transporter located on the tonoplast, and so these results are consistent with an important role for vacuolar Ca2+ transport in the signaling systems that trigger flooding response.
ISSN:1469-8137
DOI:10.1111/nph.19274