MiR-122 knockdown regulates vascular smooth muscle cells phenotypic switching through enhanced FOXO3 expression

Vascular smooth muscle cells (VSMCs) phenotypic switching is identified as enhanced dedifferentiation, proliferation, and migration ability of VSMCs, in which microRNAs have been identified as important regulators of the process. The present study is aimed to explore the pathophysiological effect of...

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Published in:Canadian journal of physiology and pharmacology 2023-12, Vol.101 (12), p.652-660
Main Authors: Zhang, Lan, Huang, Qian-wei, Pu, Yan-fen, Xiao, Xiao-qiang, Song, Bian- jing, Zhang, Xue-ping, Yang, Yong-sheng, Zhang, Yu- song, Gong, Fu-han
Format: Article
Language:English
Subjects:
Cell proliferation
FOXO3 protein
Gene expression
Genetic regulation
Health aspects
MicroRNA
miRNA
Platelet-derived growth factor
Platelet-derived growth factor BB
Smooth muscle
Therapeutic targets
Transcription factors
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Summary:Vascular smooth muscle cells (VSMCs) phenotypic switching is identified as enhanced dedifferentiation, proliferation, and migration ability of VSMCs, in which microRNAs have been identified as important regulators of the process. The present study is aimed to explore the pathophysiological effect of miR-122 on VSMC phenotypic modulation. Here, the result showed that the decreased miR-122 expression was found in VSMCs subjected to platelet- derived growth factor-BB (PDGF-BB) treatment. Next, we investigated the response of miR-122 knockdown in VSMCs with PDGF-BB stimulation. MiR-122 silencing showed increased proliferation and migration capability, whereas attenuated the differentiation markers expression. The above results were reversed by miR-122 overexpression. Finally, we further demonstrated that FOXO3 was an important target for miR- 122. Collectively, we demonstrated that miR-122 silencing promoted VSMC phenotypic modulation partially through upregulated FOXO3 expression that indicated miR-122 maybe a novel therapeutic target for neointimal formation.
ISSN:0008-4212
1205-7541
DOI:10.1139/cjpp-2022-0549