Green Synthesized Zinc Oxide Nanoparticles Attenuate Acrylamide-Induced Cardiac Injury via Controlling Endoplasmic Reticulum Stress–Associated Apoptosis Through ATF3/CHOP/BCL2 Signaling in Rats

• Published in: Biological trace element research 2024-06, Vol.202 (6), p.2657-2671
• Main Authors: Alosaimi, Manal, Abd-Elhakim, Yasmina M., Mohamed, Amany Abdel-Rahman, Metwally, Mohamed M. M., Khamis, Tarek, Alansari, Wafa S., Eskandrani, Areej A., Essawi, Walaa M., Awad, Marwa M., El-Shaer, Rehab A. A., Ibrahim, Sarah, Nassan, Mohamed A., Metwally, Aya Sh
• Format: Article
• Language: English
• Subjects: Acrylamide; Acrylamide - toxicity; Activating transcription factor 3; Activating Transcription Factor 3 - metabolism; Animals; Antioxidants; Apoptosis; Apoptosis - drug effects; Bcl-2 protein; Biochemistry; Biological stress; Biomedical and Life Sciences; Biotechnology; Caspase-3; Damage; Distilled water; Endoplasmic reticulum; Endoplasmic Reticulum Stress - drug effects; Gene expression; Genes; Green Chemistry Technology; Heart; Heart Injuries - chemically induced; Heart Injuries - drug therapy; Heart Injuries - metabolism; Heart Injuries - pathology; Heart Injuries - prevention & control; Heavy metals; Histopathology; Indicators; Life Sciences; Male; Metal Nanoparticles - chemistry; Metal oxides; Nanoparticles; Nanoparticles - chemistry; Nutrition; Oncology; Oral administration; Oxidative stress; Oxidative Stress - drug effects; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction - drug effects; Synthesis; Toxicity; Transcription Factor CHOP - metabolism; Zinc; Zinc oxide; Zinc Oxide - chemistry; Zinc Oxide - pharmacology; Zinc oxides
• ISSN: 0163-4984; 1559-0720; 1559-0720
• DOI: 10.1007/s12011-023-03855-1

Due to their distinctive properties, several eco-friendly metal oxide nanoparticles were assessed for their possible cardioprotective properties. Acrylamide (ACD), a pervasive chemical in food and the environment, has been linked to cardiac toxicity. Therefore, this study examined the probable protective effect of green synthesized zinc oxide nanoparticles (GS-ZNPs) against ACD-oral exposure–induced cardiac damage in rats. For 60 days, 40 male Sprague-Dawley rats were separated into four sets that orally administered distilled water, 10-mg GS-ZNP/kg b.w., 20-mg ACD/kg b.w., or GS-ZNP + ACD. Then, cardiac damage indicators comprising CPK, CK-MB, cTn, and LDH were assessed. Besides, cardiac tissues’ architecture, oxidative stress indicators, and Zn content were evaluated. The mRNA expression of the ERS-related genes, including ATF3, ATF4, ATF6, XBP-1, CHOP, JNKs, and BiP, were determined. Moreover, ERS-dependent anti-apoptotic (BCL-2) and apoptotic (Caspase-3 and BAX) genes mRNA expression were analyzed. The results showed that GS-ZNP significantly alleviated the increased ACD-induced serum cardiac damage indicators, MDA tissue content, and histopathological changes. Furthermore, the ACD-induced reduction of antioxidants and Zn heart contents were significantly reestablished by GS-ZNP. Furthermore, the ACD-induced upregulation of the ERS-encoding genes and apoptotic genes was reversed by GS-ZNP. Besides, the ACD-induced BCL-2 downregulation was counteracted by GS-ZNP. Overall, GS-ZNP could be a biologically potent compound to alleviate ACD’s cardiotoxic effects, possibly by controlling the ERS and apoptosis-related genes and antioxidant activity.