Cycloartane-type triterpenoids and steroids from Trichilia connaroides and their multidrug resistance reversal activities

• Published in: Phytochemistry (Oxford) 2023-12, Vol.216, p.113867-113867, Article 113867
• Main Authors: Wang, Sibei, Shi, Zhaoyu, Zhang, Han, Hou, Jiantong, Lee, Dongho, Xu, Jing, Guo, Yuanqiang
• Format: Article
• Language: English
• Subjects: Cycloartane-type triterpenoids; Meliaceae; Multidrug resistance reversal activities; Steroids; Trichilia connaroides
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2023.113867

Four undescribed cycloartane-type triterpenoids (1−4) and seven undescribed steroids (6−12), along with five known analogues (5 and 13−16), were isolated from the leaves of Trichilia connaroides. Their structures were identified based on the NMR data and HRESIMS, and the absolute configurations were determined through single-crystal X-ray diffraction analysis, Mosher's method, and ECD calculations. The multidrug resistance (MDR) reversal activities of all the isolates were assessed, and compounds 10 and 11 showed significant activities to reverse the MDR of MCF-7/DOX cells with IC50 values of 2.90 and 3.76 μM, respectively. These bioactive compounds may bring fresh insights into the research and development of MDR reversal agents. Eleven undescribed compounds were isolated from Trichilia connaroides. Some of them exhibited multidrug resistance reversal activities in MCF-7/DOX cell line. [Display omitted] •Eleven undescribed compounds were isolated from Trichilia connaroides.•Their structures were confirmed by ECD, X-ray diffraction, and Mosher's methods.•Compounds 10 and 11 showed significant activity to reverse MDR in MCF-7/DOX cells.