Calcium regulates primary nitrate response associated gene transcription in a time- and dose-dependent manner

Nitrate (NO 3 − ) is the primary source of nitrogen preferred by most arable crops, including wheat. The pioneering experiment on primary nitrate response (PNR) was carried out three decades ago. Since then, much research has been carried out to understand the NO 3 − signaling. Nitrate is sensed by...

Full description

Saved in:
Bibliographic Details
Published in:Protoplasma 2024-03, Vol.261 (2), p.257-269
Main Authors: Adavi, Sandeep B., Sathee, Lekshmy
Format: Article
Language:English
Subjects:
Arabidopsis - metabolism
Biomedical and Life Sciences
Calcium - metabolism
Calcium nitrate
Cell Biology
Gene Expression Regulation, Plant
Gene regulation
Kinases
Life Sciences
Localization
Nitrate reductase
Nitrates
Nitrates - metabolism
Nitrogen - metabolism
Original Article
Plant Sciences
Transcription, Genetic
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitrate (NO 3 − ) is the primary source of nitrogen preferred by most arable crops, including wheat. The pioneering experiment on primary nitrate response (PNR) was carried out three decades ago. Since then, much research has been carried out to understand the NO 3 − signaling. Nitrate is sensed by the dual affinity NO 3 − transceptor NPF6.3, which further relays the information to a master regulator NIN-like protein 7 (NLP7) through calcium-dependent protein kinases (CPK10, CPK30, CPK32), highlighting the importance of calcium ion (Ca 2+ ) as one of the important secondary messengers in relaying the NO 3 − signaling in Arabidopsis. In a previous study, we found that Ca 2+ regulates nitrogen starvation response in wheat. In this study, 10 days old NO 3 − -starved wheat seedlings were exposed to various treatments. Our study on time course changes in expression of PNR sentinel genes; NPF6.1 , NPF6.2 , NRT2.1 , NRT2.3 , NR , and NIR in wheat manifest the highest level of expression at 30 min after NO 3 − exposure. The use of Ca 2+ chelator EGTA confirmed the involvement of Ca 2+ in the regulation of transcription of NPF s and NRT s as well the NO 3 − uptake. We also observed the NO 3 − dose-dependent and tissue-specific regulation of nitrate reductase activity involving Ca 2+ as a mediator. The participation of Ca 2+ in the PNR and NO 3 − signaling in wheat is confirmed by pharmacological analysis, physiological evidences, and protoplast-based Ca 2+ localization.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-023-01893-z