Alpha-Fetoprotein as a Factor of Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells

We studied the role of alpha-fetoprotein (AFP) in regulation of differentiation and functional activity of human myeloid-derived suppressor cells (MDSC) in vitro . To obtain MDSC, CD11b + cells were isolated from the peripheral blood of healthy donors followed by cytokine induction (IL-1β+GM-CSF) in...

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Published in:Bulletin of experimental biology and medicine 2023-08, Vol.175 (4), p.535-543
Main Authors: Shardina, K. Yu, Zamorina, S. A., Timganova, V. P., Bochkova, M. S., Uzhviyuk, S. V., Chereshnev, V. A.
Format: Article
Language:English
Subjects:
APRIL protein
Arginase
Biomedical and Life Sciences
Biomedicine
CD11b antigen
Cell Biology
Cytokines
Gelatinase A
Glycoproteins
Granulocyte-macrophage colony-stimulating factor
Internal Medicine
Laboratory Medicine
Monocytes
Pathology
Peripheral blood
Phenotypes
Pregnancy
Suppressor cells
Tryptophan 2,3-dioxygenase
α-Fetoprotein
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Summary:We studied the role of alpha-fetoprotein (AFP) in regulation of differentiation and functional activity of human myeloid-derived suppressor cells (MDSC) in vitro . To obtain MDSC, CD11b + cells were isolated from the peripheral blood of healthy donors followed by cytokine induction (IL-1β+GM-CSF) into the MDSC phenotype. The cell functions were assessed by the expression of indoleamine 2,3-dioxygenase (IDO) and arginase-1 (Arg1) and cytokine profile of the cell cultures. Native AFP did not affect the total number of MDSC and the percentage of polymorphonuclear MDSC (PMN-MDSC), but increased the number of monocytic MDSC (M-MDSC). AFP did not change the expression of Arg1, but in low concentrations (10 and 50 U/ml) increased the number of IDO-containing cells. AFP modulated the cytokine profile of CD11b + cells: it reliably decreased the level of IL-19 (50 and100 U/ml) and showed a tendency to decrease the levels of IL-34, MMP-2, sCD163, CHI3L1, OPN and to increase the levels of IL-29, IL-32, APRIL, PTX3, and sTNF-R1. Thus, we have demonstrated a regulatory effect of native AFP at the level of MDSC generated from CD11b + cells under conditions of cytokine induction in vitro .
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-023-05901-3