Multi-omics analysis to reveal key pathways involved in low C/N ratio stress response in Pseudomonas sp. LW60 with superior nitrogen removal efficiency

[Display omitted] •A multi-omics approach was first used to explore stress resistance mechanisms.•Pseudomonas sp. LW60 removed 100 % nitrogen at C/N 10 and 4 °C.•High ammonia nitrogen removal efficiency (81.31 %) at C/N 

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Published in:Bioresource technology 2023-12, Vol.389, p.129812-129812, Article 129812
Main Authors: Wang, Li, Wang, Shipeng, Chen, Chen, Tang, Yueqin, Liu, Baicang
Format: Article
Language:English
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Low C/N ratio
Metabolome
Nitrogen removal bacteria
Proteome
Transcriptome
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cited_by cdi_FETCH-LOGICAL-c345t-931c330d585a7579e827ac7389fb22ae8b0a24b7783cbbe69e719755a20c605f3
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creator Wang, Li
Wang, Shipeng
Chen, Chen
Tang, Yueqin
Liu, Baicang
description [Display omitted] •A multi-omics approach was first used to explore stress resistance mechanisms.•Pseudomonas sp. LW60 removed 100 % nitrogen at C/N 10 and 4 °C.•High ammonia nitrogen removal efficiency (81.31 %) at C/N 
doi_str_mv 10.1016/j.biortech.2023.129812
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LW60 with superior nitrogen removal efficiency</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Wang, Li ; Wang, Shipeng ; Chen, Chen ; Tang, Yueqin ; Liu, Baicang</creator><creatorcontrib>Wang, Li ; Wang, Shipeng ; Chen, Chen ; Tang, Yueqin ; Liu, Baicang</creatorcontrib><description>[Display omitted] •A multi-omics approach was first used to explore stress resistance mechanisms.•Pseudomonas sp. LW60 removed 100 % nitrogen at C/N 10 and 4 °C.•High ammonia nitrogen removal efficiency (81.31 %) at C/N &lt; 6 and 4 °C.•Central carbon and amino acid pathways were enhanced under low C/N ratio stress. In practical engineering, nitrogen removal at low temperatures or low C/N ratios is difficult. Although strains can remove nitrogen well at low temperatures, there is no research on the performance and deep mechanism of strains under low C/N ratio stress. In this study, Pseudomonas sp. LW60 with superior nitrogen removal efficiency under low C/N ratio stress was isolated at 4 °C. With a C/N ratio of 2–10, the NH4+-N removal efficiency was 40.02 %-100 % at 4 °C. Furthermore, the resistance mechanism of Pseudomonas sp. LW60 to low C/N ratio stress was deeply investigated by multi-omics. The results of transcriptome, proteome, and metabolome revealed that the resistance of strain LW60 to low C/N ratio stress was attributed to enhanced central carbon metabolism, amino acid metabolism, and ABC transporters, rather than nitrogen removal pathways. 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LW60 with superior nitrogen removal efficiency</title><title>Bioresource technology</title><description>[Display omitted] •A multi-omics approach was first used to explore stress resistance mechanisms.•Pseudomonas sp. LW60 removed 100 % nitrogen at C/N 10 and 4 °C.•High ammonia nitrogen removal efficiency (81.31 %) at C/N &lt; 6 and 4 °C.•Central carbon and amino acid pathways were enhanced under low C/N ratio stress. In practical engineering, nitrogen removal at low temperatures or low C/N ratios is difficult. Although strains can remove nitrogen well at low temperatures, there is no research on the performance and deep mechanism of strains under low C/N ratio stress. In this study, Pseudomonas sp. LW60 with superior nitrogen removal efficiency under low C/N ratio stress was isolated at 4 °C. With a C/N ratio of 2–10, the NH4+-N removal efficiency was 40.02 %-100 % at 4 °C. Furthermore, the resistance mechanism of Pseudomonas sp. LW60 to low C/N ratio stress was deeply investigated by multi-omics. The results of transcriptome, proteome, and metabolome revealed that the resistance of strain LW60 to low C/N ratio stress was attributed to enhanced central carbon metabolism, amino acid metabolism, and ABC transporters, rather than nitrogen removal pathways. This study isolated a strain with low C/N ratio tolerance and deeply explored its tolerance mechanism by multi-omics.</description><subject>Low C/N ratio</subject><subject>Metabolome</subject><subject>Nitrogen removal bacteria</subject><subject>Proteome</subject><subject>Transcriptome</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFkcvO0zAQhSMEEuWHV0Beskl-X-rY3oEqblK5LEAsLceZUJckDh4nVZ6E1yWlsGY1szjfmTk6RfGc0YpRVt-fqybElMGfKk65qBg3mvEHxY5pJUpuVP2w2FFT01JLvn9cPEE8U0oFU3xX_Pow9zmUcQgeiRtdv2JAkiNJsIDryQ9YyeTy6eJWJGFcYr9Auy2kjxdyuP9IksshEswJEDcIpzgiXAWfEeY2DnF0SHCqyPFbTckl5BPBeYK0vUzGkFP8DuPGDXHZrkHXBR9g9OvT4lHneoRnf-dd8fXN6y-Hd-Xx09v3h1fH0ou9zKURzAtBW6mlU1IZ0Fw5r4Q2XcO5A91Qx_eNUlr4poHagGJGSek49TWVnbgrXtx8pxR_zoDZDgE99L0bIc5ouVbUGCmM2aT1TepTREzQ2SmFwaXVMmqvTdiz_deEvTZhb01s4MsbCFuQJUCy-CcktCGBz7aN4X8WvwHnD5fs</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Wang, Li</creator><creator>Wang, Shipeng</creator><creator>Chen, Chen</creator><creator>Tang, Yueqin</creator><creator>Liu, Baicang</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3219-1924</orcidid></search><sort><creationdate>202312</creationdate><title>Multi-omics analysis to reveal key pathways involved in low C/N ratio stress response in Pseudomonas sp. 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LW60 with superior nitrogen removal efficiency under low C/N ratio stress was isolated at 4 °C. With a C/N ratio of 2–10, the NH4+-N removal efficiency was 40.02 %-100 % at 4 °C. Furthermore, the resistance mechanism of Pseudomonas sp. LW60 to low C/N ratio stress was deeply investigated by multi-omics. The results of transcriptome, proteome, and metabolome revealed that the resistance of strain LW60 to low C/N ratio stress was attributed to enhanced central carbon metabolism, amino acid metabolism, and ABC transporters, rather than nitrogen removal pathways. This study isolated a strain with low C/N ratio tolerance and deeply explored its tolerance mechanism by multi-omics.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.biortech.2023.129812</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-3219-1924</orcidid></addata></record>
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subjects Low C/N ratio
Metabolome
Nitrogen removal bacteria
Proteome
Transcriptome
title Multi-omics analysis to reveal key pathways involved in low C/N ratio stress response in Pseudomonas sp. LW60 with superior nitrogen removal efficiency
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