Validation of mitotic harvesting method with human cervical carcinoma HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators for radiation research

The cell cycle is a series of events in the process of one cell giving rise to two daughter cells. The mitotic harvesting method, established by Terasima and Tolmach in the 1960s, causes minimal physiological stress on the cells and achieves a high degree of cell cycle synchrony by collecting only m...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical Research 2023/09/27, Vol.44(5), pp.181-186
Main Authors: SEINO, Ryosuke, UNO, Hiroto, FUKUNAGA, Hisanori
Format: Article
Language:English
Subjects:
Cell cycle
Cervical cancer
Cervical carcinoma
Fluorescence
Harvesting
HeLa cells
Image analysis
Image processing
Indicators
Radiation
Radiation tolerance
Radiosensitivity
Stress (physiology)
Ubiquitination
Versatility
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The cell cycle is a series of events in the process of one cell giving rise to two daughter cells. The mitotic harvesting method, established by Terasima and Tolmach in the 1960s, causes minimal physiological stress on the cells and achieves a high degree of cell cycle synchrony by collecting only mitotic cells from a cultured cell system. The purpose of the present study is to validate the versatility of the mitotic harvesting method using human cervical cell line HeLa cells expressing Fluorescent Ubiquitination-based Cell Cycle Indicators (FUCCI) and to estimate the cell cycle-dependent changes in radiosensitivity in HeLa–FUCCI cells. The image analysis showed that cell cycle synchrony was maintained for at least 24 hours after mitotic cell collection. Also, the clonogenic assay demonstrated changes in radiosensitivity that were cell cycle dependent. These results indicate that the mitotic harvesting method using FUCCI-expressing cells has high versatility in the field of radiation cell biology.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.44.181