Tracing of Helicobacter pylori in the middle ear and mastoid mucosa of patients under 18 years of age with chronic otitis media (with and without cholesteatomas)

It has been estimated that about 5 million people of those affected with otitis media have cholesteatoma, however, its pathophysiology is unclear. In this study we aimed to detect via polymerase chain reaction and real-time polymerase chain reaction in young patients with chronic otitis media. Patie...

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Bibliographic Details
Published in:Journal of laryngology and otology 2024-05, Vol.138 (5), p.503-506
Main Authors: González-Eslait, Francisco Javier, Blanco-Sarmiento, Pedro Abel, Bejarano-Domínguez, Kabethliz, Barreto, José María, Ruiz-Tejada, Emelina
Format: Article
Language:English
Subjects:
Adolescent
Child
Cholesteatoma, Middle Ear - microbiology
Chronic Disease
Cross-Sectional Studies
Ear diseases
Ear, Middle - microbiology
Female
Gastroesophageal reflux
Hearing loss
Helicobacter Infections - complications
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Humans
Inflammation
Laboratories
Main Article
Male
Mastoid - microbiology
Middle ear
Mucosa
Mucous Membrane - microbiology
Otitis media
Otitis Media - microbiology
Otolaryngology
Polymerase chain reaction
Prospective Studies
Real-Time Polymerase Chain Reaction
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Summary:It has been estimated that about 5 million people of those affected with otitis media have cholesteatoma, however, its pathophysiology is unclear. In this study we aimed to detect via polymerase chain reaction and real-time polymerase chain reaction in young patients with chronic otitis media. Patients included in our prospective cross-sectional study had middle-ear/mastoid inflammation and underwent surgical procedures. Middle-ear mucosa samples were collected, and genomic DNA was extracted for detection by polymerase chain reaction and real-time polymerase chain reaction analyses. Sociodemographic data and gastroesophageal reflux symptoms were analysed. We included 49 patients with mean age of 12.7 ± 3.8 years. Twenty per cent of the patients were diagnosed with cholesteatoma. No increase in -amplified fluorescence was observed, indicating absence of . Due to the absence of amplification for and the fact that albumin was amplified in all samples, we conclude that does not appear to be a causal factor.
ISSN:0022-2151
1748-5460
DOI:10.1017/S002221512300169X