Enhancement of bio-S0 recovery and revealing the inhibitory effect on microorganisms under high sulfide loading

• Published in: Environmental research 2023-12, Vol.238, p.117214-117214, Article 117214
• Main Authors: Wang, Junjie, Cheng, Zhuowei, Wang, Jiade, Chen, Dongzhi, Chen, Jianmeng, Yu, Jianming, Qiu, Songkai, Dionysiou, Dionysios D.
• Format: Article
• Language: English
• Subjects: Burkholderiaceae; carbon sequestration; DNA damage; Meta-omics; metagenomics; NAD(P)H dehydrogenase (quinone); oxidation; reactive oxygen species; S0 natural settlement; Solution reflux; Sulfide oxidation; sulfides; transcriptomics
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2023.117214

Biodesulfurization is a mature technology, but obtaining biosulfur (S0) that can be easily settled naturally is still a challenge. Increasing the sulfide load is one of the known methods to obtain better settling of S0. However, the inhibitory effect of high levels of sulfide on microbes has also not been well studied. We constructed a high loading sulfide (1.55–10.86 kg S/m3/d) biological removal system. 100% sulfide removal and 0.56–2.53 kg S/m3/d S0 (7.0 ± 0.09–16.4 ± 0.25 μm) recovery were achieved at loads of 1.55–7.75 kg S/m3/d. Under the same load, S0 in the reflux sedimentation tank, which produced larger S0 particles (24.2 ± 0.73–53.8 ± 0.70 μm), increased the natural settling capacity and 45% recovery. For high level sulfide inhibitory effect, we used metagenomics and metatranscriptomics analyses. The increased sulfide load significantly inhibited the expression of flavin cytochrome c sulfide dehydrogenase subunit B (fccB) (Decreased from 615 ± 75 to 30 ± 5 TPM). At this time sulfide quinone reductase (SQR) (324 ± 185–1197 ± 51 TPM) was mainly responsible for sulfide oxidation and S0 production. When the sulfide load reached 2800 mg S/L, the SQR (730 ± 100 TPM) was also suppressed. This resulted in the accumulation of sulfide, causing suppression of carbon sequestration genes (Decreased from 3437 ± 842 to 665 ± 175 TPM). Other inhibitory effects included inhibition of microbial respiration, production of reactive oxygen species, and DNA damage. More sulfide-oxidizing bacteria (SOB) and newly identified potential SOB (99.1%) showed some activity (77.6%) upon sulfide accumulation. The main microorganisms in the sulfide accumulation environment were Thiomicrospiracea and Burkholderiaceae, whose sulfide oxidation capacity and respiration were not significantly inhibited. This study provides a new approach to enhance the natural sedimentation of S0 and describes new microbial mechanisms for the inhibitory effects of sulfide. [Display omitted] •High sulfide concentration facilitates S0 recovery.•Sedimentation tank reflux S0 can increase the natural settling capacity of S0.•High levels of sulfide have an inhibitory effect on metabolism and respiration.•More species of sulfide oxidizing bacteria against high levels of sulfide toxicity.•Thiomicrospiracea and Burkholderiaceae are able to tolerate sulfide toxicity.