The wild solitary bees Andrena vaga, Anthophora plumipes, Colletes cunicularius, and Osmia cornuta microbiota are host specific and dominated by endosymbionts and environmental microorganisms

We characterized the microbial communities of the crop, midgut, hindgut, and ovaries of the wild solitary bees Andrena vaga , Anthophora plumipes , Colletes cunicularius , and Osmia cornuta through 16S rRNA gene and ITS2 amplicon sequencing and a large-scale isolation campaign. The bacterial communi...

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Bibliographic Details
Published in:Microbial ecology 2023-11, Vol.86 (4), p.3013-3026
Main Authors: Hettiarachchi, Amanda, Cnockaert, Margo, Joossens, Marie, Gekière, Antoine, Meeus, Ivan, Vereecken, Nicolas J., Michez, Denis, Smagghe, Guy, Vandamme, Peter
Format: Article
Language:English
Subjects:
Andrena vaga
Animals
Anthophora plumipes
Bacteria
Bees
Biomedical and Life Sciences
Colletes cunicularius
Ecology
Endosymbionts
Geoecology/Natural Processes
Hindgut
Life Sciences
Microbial activity
Microbial Ecology
Microbiology
Microbiota
Microorganisms
Midgut
Mycobiome
Nature Conservation
Osmia cornuta
Ovaries
Redundancy
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sampling
Spiroplasma
Symbionts
Water Quality/Water Pollution
Yeasts
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Summary:We characterized the microbial communities of the crop, midgut, hindgut, and ovaries of the wild solitary bees Andrena vaga , Anthophora plumipes , Colletes cunicularius , and Osmia cornuta through 16S rRNA gene and ITS2 amplicon sequencing and a large-scale isolation campaign. The bacterial communities of these bees were dominated by endosymbionts of the genera Wolbachia and Spiroplasma . Bacterial and yeast genera representing the remaining predominant taxa were linked to an environmental origin. While only a single sampling site was examined for Andrena vaga , Anthophora plumipes , and Colletes cunicularius , and two sampling sites for Osmia cornuta , the microbiota appeared to be host specific: bacterial, but not fungal, communities generally differed between the analyzed bee species, gut compartments and ovaries. This may suggest a selective process determined by floral and host traits. Many of the gut symbionts identified in the present study are characterized by metabolic versatility. Whether they exert similar functionalities within the bee gut and thus functional redundancy remains to be elucidated.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-023-02304-9