Altered 5-methylcytosine modification of mRNA is involved in the pathogenesis of pre-eclampsia

5-Methylcytosine (m C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m C peaks a...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2023-11, Vol.124 (11), p.1720-1733
Main Authors: Tong, Jing, Li, Hongwanyu, Zhang, Cong
Format: Article
Language:English
Subjects:
5-Methylcytosine - metabolism
Bioinformatics
Decidua
Eclampsia
Encyclopedias
Female
Gene expression
Gene sequencing
Genes
Genomes
Humans
Immunoprecipitation
Pathogenesis
Pre-Eclampsia - pathology
Preeclampsia
Pregnancy
Reproducibility of Results
Ribonucleic acid
RNA
RNA modification
RNA, Messenger - genetics
Signal Transduction
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Summary:5-Methylcytosine (m C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m C peaks and mRNA expression profile in the decidua of human early-onset PE (EPE), late-onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP-seq and RNA-seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.30479