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Altered 5-methylcytosine modification of mRNA is involved in the pathogenesis of pre-eclampsia
5-Methylcytosine (m C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m C peaks a...
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| Published in: | Journal of cellular biochemistry 2023-11, Vol.124 (11), p.1720-1733 |
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| container_title | Journal of cellular biochemistry |
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| creator | Tong, Jing Li, Hongwanyu Zhang, Cong |
| description | 5-Methylcytosine (m
C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m
C peaks and mRNA expression profile in the decidua of human early-onset PE (EPE), late-onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP-seq and RNA-seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m
C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m
C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m
C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE. |
| doi_str_mv | 10.1002/jcb.30479 |
| format | article |
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C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m
C peaks and mRNA expression profile in the decidua of human early-onset PE (EPE), late-onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP-seq and RNA-seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m
C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m
C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m
C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.30479</identifier><identifier>PMID: 37796115</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>5-Methylcytosine - metabolism ; Bioinformatics ; Decidua ; Eclampsia ; Encyclopedias ; Female ; Gene expression ; Gene sequencing ; Genes ; Genomes ; Humans ; Immunoprecipitation ; Pathogenesis ; Pre-Eclampsia - pathology ; Preeclampsia ; Pregnancy ; Reproducibility of Results ; Ribonucleic acid ; RNA ; RNA modification ; RNA, Messenger - genetics ; Signal Transduction</subject><ispartof>Journal of cellular biochemistry, 2023-11, Vol.124 (11), p.1720-1733</ispartof><rights>2023 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c273t-62d879cae30f3dd273448808367d0ff94a5493066459b8424923f4b106f1f3103</cites><orcidid>0000-0002-9650-4398</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37796115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tong, Jing</creatorcontrib><creatorcontrib>Li, Hongwanyu</creatorcontrib><creatorcontrib>Zhang, Cong</creatorcontrib><title>Altered 5-methylcytosine modification of mRNA is involved in the pathogenesis of pre-eclampsia</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>5-Methylcytosine (m
C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m
C peaks and mRNA expression profile in the decidua of human early-onset PE (EPE), late-onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP-seq and RNA-seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m
C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m
C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m
C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE.</description><subject>5-Methylcytosine - metabolism</subject><subject>Bioinformatics</subject><subject>Decidua</subject><subject>Eclampsia</subject><subject>Encyclopedias</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Pathogenesis</subject><subject>Pre-Eclampsia - pathology</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Reproducibility of Results</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA modification</subject><subject>RNA, Messenger - genetics</subject><subject>Signal Transduction</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpdkF1LwzAUhoMobk4v_ANS8EYvOk8-mjSXY_gFQ0H01pKlictom9q0g_17o5teeHXgnOd9OTwInWOYYgBys9bLKQUm5AEaY5AiZZyxQzQGQSElFJMROglhDQBSUnKMRlQIyTHOxuh9VvWmM2WSpbXpV9tKb3sfXGOS2pfOOq1655vE26R-eZolLiSu2fhqExOuSfqVSVrVr_yHaUyIx8i1nUmNrlTdBqdO0ZFVVTBn-zlBb3e3r_OHdPF8_zifLVJNBO1TTspcSK0MBUvLMu4Yy3PIKRclWCuZypikwDnL5DJnhElCLVti4BZbioFO0NWut-3852BCX9QuaFNVqjF-CAXJBSUZIUAjevkPXfuha-J3kZIEC55FSxN0vaN050PojC3aztWq2xYYim_pRZRe_EiP7MW-cVjWpvwjfy3TL3hteqk</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Tong, Jing</creator><creator>Li, Hongwanyu</creator><creator>Zhang, Cong</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9650-4398</orcidid></search><sort><creationdate>20231101</creationdate><title>Altered 5-methylcytosine modification of mRNA is involved in the pathogenesis of pre-eclampsia</title><author>Tong, Jing ; Li, Hongwanyu ; Zhang, Cong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c273t-62d879cae30f3dd273448808367d0ff94a5493066459b8424923f4b106f1f3103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>5-Methylcytosine - metabolism</topic><topic>Bioinformatics</topic><topic>Decidua</topic><topic>Eclampsia</topic><topic>Encyclopedias</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genomes</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Pathogenesis</topic><topic>Pre-Eclampsia - pathology</topic><topic>Preeclampsia</topic><topic>Pregnancy</topic><topic>Reproducibility of Results</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA modification</topic><topic>RNA, Messenger - genetics</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tong, Jing</creatorcontrib><creatorcontrib>Li, Hongwanyu</creatorcontrib><creatorcontrib>Zhang, Cong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tong, Jing</au><au>Li, Hongwanyu</au><au>Zhang, Cong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered 5-methylcytosine modification of mRNA is involved in the pathogenesis of pre-eclampsia</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>124</volume><issue>11</issue><spage>1720</spage><epage>1733</epage><pages>1720-1733</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>5-Methylcytosine (m
C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre-eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-sequencing (RNA-seq) to map m
C peaks and mRNA expression profile in the decidua of human early-onset PE (EPE), late-onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP-seq and RNA-seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m
C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m
C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m
C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37796115</pmid><doi>10.1002/jcb.30479</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-9650-4398</orcidid></addata></record> |
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| ispartof | Journal of cellular biochemistry, 2023-11, Vol.124 (11), p.1720-1733 |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | 5-Methylcytosine - metabolism Bioinformatics Decidua Eclampsia Encyclopedias Female Gene expression Gene sequencing Genes Genomes Humans Immunoprecipitation Pathogenesis Pre-Eclampsia - pathology Preeclampsia Pregnancy Reproducibility of Results Ribonucleic acid RNA RNA modification RNA, Messenger - genetics Signal Transduction |
| title | Altered 5-methylcytosine modification of mRNA is involved in the pathogenesis of pre-eclampsia |
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