Biochemical and spectroscopic studies of the response of Convolvulus arvensis L. to chromium(III) and chromium(VI) stress

• Published in: Environmental toxicology and chemistry 2006-01, Vol.25 (1), p.220-226
• Main Authors: Montes-Holguin, Milka O., Peralta-Videa, Jose R., Meitzner, George, Martinez-Martinez, Alejandro, de la Rosa, Guadalupe, Castillo-Michel, Hiram A., Gardea-Torresdey, Jorge L.
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Applied ecology; Ascorbate Peroxidases; Biochemistry; Biological and medical sciences; Catalase; Catalase - metabolism; Catalytic oxidation; Chromium; Chromium - analysis; Chromium - toxicity; Chromium(III); Chromium(VI); Convolvulus - drug effects; Convolvulus - enzymology; Convolvulus - metabolism; Convolvulus arvensis; Ecotoxicology, biological effects of pollution; Emission spectroscopy; Enzymes; Flowers & plants; Fundamental and applied biological sciences. Psychology; General aspects; Hydroponics; Oxidation-Reduction; Oxidative Stress; Peroxidase; Peroxidases - metabolism; Plant Leaves - chemistry; Plant Roots - chemistry; Plant Roots - enzymology; Plant Shoots - enzymology; Plant Stems - chemistry; Plant tissues; Roots; Shoots; Speciation; Spectrum Analysis; X-Rays
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1897/05-089R.1

The objective of the present study was to determine the oxidative stress caused by hexavalent chromium (Cr[VI]), the chromium (Cr) uptake, and the Cr speciation in Convolvulus arvensis L. plants grown in hydroponics media containing either Cr(VI) or Cr(III). The results demonstrated that C. arvensis plants exposed to Cr(VI) concentrations ranging from 0 to 40 mg/L expressed higher ascorbate peroxidase specific activity in roots than in shoots. On the other hand, catalase activity monitored in plants exposed to 2 mg/L of Cr(VI) for 24 h increased in roots after a few hours of exposure. However, catalase activity in shoots revealed a decrement almost immediately after treatment was initiated. The results from x‐ray absorption spectroscopic studies indicated that the oxidation state of the supplied Cr(III) remained the same in plant tissues. The supplied Cr(VI), however, was reduced to the trivalent form in plant tissues. The results of inductively coupled plasma/optical emission spectroscopy demonstrated that after 5 d, the roots of plants exposed to 40 mg/L of Cr(III) or Cr(VI) accumulated approximately 25,000 and 3,500 mg/kg dry weight of Cr, respectively. Nevertheless, shoots concentrated 1,500 and 2,000 mg/kg dry weight of Cr from Cr(III) and Cr(VI), respectively, which indicated that Cr moved faster into C. arvensis plants when supplied as Cr(VI).