Noise exposure increase apoptosis in the hippocampus of AD mice through the upregulation of CTSS

• Published in: Biochemical and biophysical research communications 2023-11, Vol.681, p.283-290
• Main Authors: Zhou, Zan, Jiang, Wen-jun, Li, Li, Si, Jun-qiang
• Format: Article
• Language: English
• Subjects: Apoptosis; APP/PS1 mouse; Cathepsin S; Hippocampus; Proteomics
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2023.09.076

In recent years, it has become an acknowledged fact that noise exposure can lead to cognitive impairments, and researchers have shown increasing interest in this area. However, the detrimental impact of noise exposure on Alzheimer's disease (AD) animal models might be considerably greater than on ordinary model mice, yet the mechanisms by which noise exposure affects the hippocampus in these models have been scarcely investigated. This study we used 4D Label-free proteomics to identify distinctive differentially expressed proteins in the hippocampus of AD model mice following noise exposure. Among these proteins, the presence of Cathepsin S(CTSS) cannot be disregarded. Utilizing experimental techniques such as Western blot, immunofluorescence, and rt-qPCR, we confirmed the expression of CTSS in the hippocampus of APP/PS1 mice after noise exposure. Additionally, we examined downstream molecules including P53,BCL-2, BAX, and CASPASE3 using KEGG pathway analysis. The results indicated an elevation in CTSS expression, a reduction in the anti-apoptotic gene BCL-2, and an increase in the expression of BAX and cleaved CASPASE3. Based on these findings, we hypothesize that noise exposure potentially heightens apoptosis within the hippocampus through upregulating CTSS expression, subsequently posing a threat to AD model animals. •This study represents the inaugural utilization of 4D label-free proteomics to explore the hippocampus changes in noise-exposed APP/PS1 mice.•Using 4D label-free proteomics, we found increased levels of CTSS protein in the hippocampus of noise-exposed APP/PS1 mice.•We have observed a link between CTSS protein upregulation and heightened hippocampal apoptosis in noise-exposed APP/PS1 mice.