Dynamic headspace GC-MS/MS analysis of ethylene oxide and 2-chloroethanol in dry food commodities: a novel approach

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Pesticides, food contaminants, and agricultural wastes, 2023, Vol.58 (11), p.659-670
• Main Authors: Nerpagar, Apurva, Langade, Nagnath, Patil, Reshma, Chiplunkar, Sanket, Kelkar, Jitendra, Banerjee, Kaushik
• Format: Article
• Language: English
• Subjects: 2-chloroethanol; Acetaldehyde; Acetonitrile; Analytical chemistry; Commodities; dry food and food additive matrices; dynamic headspace-GC-MS/MS; Ethylene oxide; Food; Ginger; Guar gum; Gums; Headspace; Locust bean gum; Metabolites; Powder; residue analysis; Residues; Seeds; Solid phases; Sorbents
• ISSN: 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2023.2264740

With frequent RASFF notifications from the EU countries, the residue testing of ethylene oxide (EtO) and its metabolite 2-chloroethanol (2-CE) in food commodities has become essential to check their compliance with MRLs. This study, for the first time, aimed at establishing a dynamic headspace-GC-MS/MS method for the simultaneous determination of these two analytes in acetonitrile extracts of cumin, ashwagandha, chilli powder, turmeric powder, guar gum, locust bean gum, and ginger powder. The samples (4 g) were extracted using acetonitrile (10 mL). A dispersive-solid phase extraction cleanup step with primary secondary amine sorbent (50 mg/mL) reduced the interfering signal of (matrix-derived) acetaldehyde by >40% in chilli powder, ginger, turmeric, and guar gum. This cleanup was not required for sesame seeds. With high selectivity and sensitivity, the GC-MS/MS approach identified and quantified both compounds simultaneously. At the spiking levels of 0.01, 0.02, and 0.05 mg/kg, the recoveries and precision were satisfactory (70-120%, RSDs, ≤15%). The headspace method-performance was similar to liquid injections. The method provided reproducible results when evaluated by two different laboratories. The method provided high-precision results for incurred residue analysis. Given its efficiency, the validated method is anticipated to improve the effectiveness of monitoring of EtO residues in food commodities.