Magnetic resonance imaging of pancreatic islets using tissue–adhesive particles containing iron oxide nanoparticles

Post–transplantation tracking of pancreatic islets is a prerequisite for advancing cell therapy to treat type 1 diabetes. Magnetic resonance imaging (MRI) has emerged as a safe and non–invasive technique for visualizing cells in clinical applications. In this study, we proposed a novel MRI contrast...

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Bibliographic Details
Published in:Journal of controlled release 2023-12, Vol.364, p.37-45
Main Authors: Le Tran, Phuong, Pham, Tung Thanh, Lee, Han Sin, Hahn, Soojung, Choi, Jeong Uk, Kim, Jae Hyeon, Jiang, Hu-Lin, Yook, Simmyung, Jeong, Jee-Heon
Format: Article
Language:English
Subjects:
Animals
Contrast agent
Diabetes Mellitus, Experimental - diagnostic imaging
Diabetes Mellitus, Experimental - metabolism
Diabetes Mellitus, Experimental - therapy
iron oxide nanoparticles
Islet transplantation
Islets of Langerhans - diagnostic imaging
Islets of Langerhans - metabolism
Islets of Langerhans Transplantation - methods
Magnetic Resonance Imaging - methods
Magnetite Nanoparticles
Mice
MRI tracking
Polymeric microspheres
Tissue Adhesives
Type 1 diabetes
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Summary:Post–transplantation tracking of pancreatic islets is a prerequisite for advancing cell therapy to treat type 1 diabetes. Magnetic resonance imaging (MRI) has emerged as a safe and non–invasive technique for visualizing cells in clinical applications. In this study, we proposed a novel MRI contrast agent formulation by encapsulating iron oxide nanoparticles (IONPs) in poly(lactic–co–glycolic acid) (PLGA) particles functionalized with a tissue adhesive polydopamine (PD) layer (IONP–PLGA–PD MS). Intriguingly, our particles facilitated efficient and robust labeling through a one–step process, allowing for the incorporation of a substantial amount of IONPs without detrimental impacts on the viability and functionality of pancreatic islets. The MRI signals emanating from islets labeled using our particles were found to be stable over 30 days in vitro and 60 days when transplanted under kidney capsules of diabetic mice. These results suggest that our approach provides a potential platform for monitoring the fate of pancreatic islets after transplantation. [Display omitted]
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2023.10.008