Diversity of Endophytic Fungi Isolated from the Bark of Ceiba pentandra (L.) Gaertn., (Bombacaceae) and Antibacterial Potential of Secalonic Acid A Produced by Diaporthe searlei EC 321

The objective of this study was to study the diversity of endophytic fungi isolated from Ceiba pentandra and to isolate their bioactive chemical compounds. The methodology used during this study consisted in isolating endophytic fungi from the bark of C. pentandra on Potato Agar. The isolates obtain...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry & biodiversity 2023-11, Vol.20 (11), p.e202301010-n/a
Main Authors: Dominique, Sagou, Alex, Pakora Gilles, Christiane, Essoh You, Dodehe, Yeo, Adèle, Kacou N'douba
Format: Article
Language:English
Subjects:
Acetic acid
Acids
Antiinfectives and antibacterials
Bacteria
Bioactive compounds
Ceiba pentandra
Chemical compounds
Diaporthe searlei
Drug resistance
E coli
Endophytes
endophytic fungi
Ethyl acetate
Fungi
Metabolites
multi-resistant bacteria
Multidrug resistance
NMR
Nuclear magnetic resonance
Ribosomal DNA
Screening
secalonic acid A
Secondary metabolites
Strains (organisms)
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this study was to study the diversity of endophytic fungi isolated from Ceiba pentandra and to isolate their bioactive chemical compounds. The methodology used during this study consisted in isolating endophytic fungi from the bark of C. pentandra on Potato Agar. The isolates obtained were identified on the basis of the ITS regions of their ribosomal DNA. Antibacterial screening of the mycelium of endophytic fungi isolated was evaluated against multidrug‐resistant E. coli and S. aureus strains. This screening led to the selection of isolates EC 321 and EC 28 for their ability to effectively inhibit the growth of the bacterial strains tested. EC 321 was grown and fermented on rice medium. Secondary metabolites were extracted with ethyl acetate. From the crude extract, secalonic acid A was isolated and identified by chromatographic and NMR. The in vitro activity of secalonic acid A against the growth of multiresistant bacterial strains was evaluated. Secalonic acid A was active against all multidrug‐resistant bacterial strains E. coli 942, E. coli 4814, S. aureus 931, S. aureus 934, S. aureus MRSA 1872 and K. pneumonia 815 with respective MICs of 18.75; 18.75; 18.75; 4.7; 37.5 and 37.5 μg/mL.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202301010