Eggplant transcription factor SmMYB5 integrates jasmonate and light signaling during anthocyanin biosynthesis

• Published in: Plant physiology (Bethesda) 2024-01, Vol.194 (2), p.1139-1165
• Main Authors: Li, Shaohang, Dong, Yanxiao, Li, Dalu, Shi, Suli, Zhao, Na, Liao, Jielei, Liu, Yang, Chen, Huoying
• Format: Article
• Language: English
• Subjects: Anthocyanins - metabolism; Basic Helix-Loop-Helix Transcription Factors - metabolism; Cyclopentanes - metabolism; Gene Expression Regulation, Plant; Oxylipins - metabolism; Solanum melongena - genetics; Solanum melongena - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1093/plphys/kiad531

Abstract Low light conditions severely suppress anthocyanin synthesis in fruit skins, leading to compromised fruit quality in eggplant (Solanum melongena L.) production. In this study, we found that exogenous methyl-jasmonate (MeJA) application can effectively rescue the poor coloration of the eggplant pericarp under low light conditions. However, the regulatory relationship between jasmonate and light signaling for regulating anthocyanin synthesis remains unclear. Here, we identified a JA response factor, SmMYB5, as an anthocyanin positive regulator by applying RNA-sequencing and characterization of transgenic plants. Firstly, we resolved that SmMYB5 can interact with TRANSPARENT TESTA8 (SmTT8), an anthocyanin-promoted BASIC HELIX-LOOP-HELIX (bHLH) transcription factor, to form the SmMYB5–SmTT8 complex and activate CHALCONE SYNTHASE (SmCHS), FLAVANONE-3-HYDROXYLASE (SmF3H), and ANTHOCYANIN SYNTHASE (SmANS) promoters by direct binding. Secondly, we revealed that JA signaling repressors JASMONATE ZIM DOMAIN5 (SmJAZ5) and SmJAZ10 can interfere with the stability and transcriptional activity of SmMYB5–SmTT8 by interacting with SmMYB5. JA can partially rescue the transcriptional activation of SmF3H and SmANS promoters by inducing SmJAZ5/10 degradation. Thirdly, we demonstrated that the protein abundance of SmMYB5 is regulated by light. CONSTITUTIVELY PHOTOMORPHOGENIC1 (SmCOP1) interacts with SmMYB5 to trigger SmMYB5 degradation via the 26S proteasome pathway. Finally, we delineated a light-dependent JA-SmMYB5 signaling pathway that promotes anthocyanin synthesis in eggplant fruit skins. These results provide insights into the mechanism of the integration of JA and light signals in regulating secondary metabolite synthesis in plants. Methyl-jasmonate promotes anthocyanin synthesis in eggplant fruit skin in a light-dependent manner.