Fabrication of layer-by-layer deposited multilayer films containing DNA and gold nanoparticle for norepinephrine biosensor

The present work describes the preparation and characterization of an electrodeposited DNA membrane doped with gold nanoparticles for the design of biosensors. The gold nanoparticles were deposited on the surface of DNA layer to build a hybrid device of nanoscale electrode array. The gold nanopartic...

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Bibliographic Details
Published in:Analytica chimica acta 2004-08, Vol.519 (2), p.161-166
Main Authors: Lu, Li-Ping, Wang, Shu-Qing, Lin, Xiang-Qin
Format: Article
Language:English
Subjects:
Analytical chemistry
Ascorbic acid
Biological and medical sciences
Biosensors
Biotechnology
Calf-thymer DNA
Chemistry
Electrochemical methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Gold nanoparticle
Methods. Procedures. Technologies
Norepinephrine
Various methods and equipments
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Summary:The present work describes the preparation and characterization of an electrodeposited DNA membrane doped with gold nanoparticles for the design of biosensors. The gold nanoparticles were deposited on the surface of DNA layer to build a hybrid device of nanoscale electrode array. The gold nanoparticles-doped DNA composite electrode was characterized by atomic force microscopy, scanning electron microscope, and electrochemistry involving electrochemical impedance spectroscopy. This electrode was successfully used for selective determination of norepinephrine (NE) in the presence of ascorbic acid (AA). The reversibility of the electrode oxidation reaction of NE is significantly improved in result of 200 mV negative shift of the voltammetric peak potential on the electrode, and a large increase in the peak current. A detection limit of 5 nM NE is obtained by using DPV in static solutions. The co-existence of a large excess of AA does not interfere with the detection. This electrode shows excellent sensitivity, good selectivity and antifouling properties.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2004.05.062