A novel molecularly imprinted electrochemical sensor for the ultrasensitive detection of tert-butylhydroquinone in edible oils

• Published in: Analytical biochemistry 2023-12, Vol.682, p.115348-115348, Article 115348
• Main Authors: Mohammed Albayatı, Safaa Hashım, Üstündağ, Zafer, Soylu, Pervin
• Format: Article
• Language: English
• Subjects: Edible oils; Food preservatives; Gold nanoparticles; Molecular imprinting techniques; Phenolic antioxidants
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2023.115348

Tert-butylhydroquinone (TBHQ) is widely used to increase the stability of food products; however, it is considered to be a highly unsafe preservative ingredient that has caused serious damage to human health. Thus, in this paper, a novel molecularly imprinted electrochemical sensor was designed for ultrasensitive, and selective detection of TBHQ in edible oils. The sensor was based on the molecularly imprinted polymer (MIP) synthesized with multiwalled carbon nanotube (MWCNT), and gold nanoparticle (GNP), as the coating materials, o-phenylenediamine (o-PDA) as the functional monomer, and TBHQ as the template molecule. The electrochemical behavior of MIP/GNP/MWCNT/GCE was studied using several electrochemical methods, which showed a low detection limit of 5 nM. Furthermore the sensor demostrated excellent stability, selectivity, repeatability, and reproducibility. It was successfully used to detect TBHQ in edible oils, with recoveries ranging from 98.44% to 102.09% and relative standard deviations (RSDs) of less than 2.16%, indicating that TBHQ detection in actual samples is both possible and accurate. Scheme 1. Schematic illustration of the modification procedure of GCE surface. [Display omitted] •MIP/GNP/MWCNT modified GCE was used as an efficient TBHQ electrochemical sensor.•Very low limit of detection 5 nM and wide linear range 0.1–0.6 μM, and 0.6–1000 μM were obtained.•Excellent selectivity, repeatability, reproducibility, and stability of the MIP sensor were observed.•Satisfactory results were observed in detecting TBHQ in black seed oil, walnut oil, and olive oil.