Analysis of Correlation Between Coronary Heart Disease and Genetic Polymorphism Detected by Gold Magnetic Nanoparticles Chromatography

It aimed to explore the correlation of Glu504Lys locus mutation of aldehyde dehydrogenase-2 (ALDH2) with coronary heart disease (CHD) based on gold magnetic nanoparticles (GMNPs) chromatography and amplification refractory mutation system-PCR (ARMS-PCR). 120 CHD patients admitted to the cardiovascul...

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Bibliographic Details
Published in:Journal of cardiovascular translational research 2024-04, Vol.17 (2), p.467-475
Main Authors: Cao, Hai-Tao, Deng, Cong-ying, Yan, Xin-min, Lin, Zhi-juan
Format: Article
Language:English
Subjects:
Aged
Aldehyde Dehydrogenase, Mitochondrial - genetics
Biomarkers - blood
Biomedical Engineering and Bioengineering
Biomedicine
Cardiology
Case-Control Studies
Chromatography
Coronary Disease - blood
Coronary Disease - diagnosis
Coronary Disease - epidemiology
Coronary Disease - genetics
Female
Gene Frequency
Genetic Association Studies
Genetic Predisposition to Disease
Gold
Human Genetics
Humans
Lipids - blood
Magnetite Nanoparticles
Male
Medicine
Medicine & Public Health
Middle Aged
Original Article
Phenotype
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Risk Assessment
Risk Factors
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Summary:It aimed to explore the correlation of Glu504Lys locus mutation of aldehyde dehydrogenase-2 (ALDH2) with coronary heart disease (CHD) based on gold magnetic nanoparticles (GMNPs) chromatography and amplification refractory mutation system-PCR (ARMS-PCR). 120 CHD patients admitted to the cardiovascular Department of Wenling First People's Hospital affiliated to Wenzhou Medical University from December 2020 to December 2021 were selected as Case group and 80 non-CHD patients admitted during the same period were selected as Ctrl group. The venous blood and indexes of Total Cholesterol (TC), Triglyceride (TG), Low Density Lipoprotein Cholesterol (LDL-C), High Density Lipoprotein Cholesterol (HDL-C), and Fasting Blood Glucose (FBS) were collected. The ARMS-PCR GMNPs chromatography based on ARMS-PCR and immunochromatography assay was adopted to detect gene polymorphism of ALDH2. Correlation between ALDH2 gene polymorphism and risk factors of CHD was analyzed via logistic regression. In contrast to Ctrl group, the genotypes of GG, GA, and AA in Case group were evidently different ( P  
ISSN:1937-5387
1937-5395
DOI:10.1007/s12265-023-10439-w