Phytoplankton interspecific interactions modified by symbiotic fungi and bacterial metabolites under environmentally relevant hydrogen peroxide concentrations stress

• Published in: Water research (Oxford) 2023-11, Vol.246, p.120739-120739, Article 120739
• Main Authors: Li, Zhe, Ma, Hua, Hong, Zhicheng, Zhang, Ting, Cao, Mingxing, Cui, Fuyi, Grossart, Hans-Peter
• Format: Article
• Language: English
• Subjects: Biodiversity; Hydrogen peroxide; Microbial metabolites; Reactive oxygen species (ROS); Water bloom
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2023.120739

•Low H2O2 concentration can promote the growth of M. aeruginosa.•Microbes play a crucial role in H2O2 regulation of phytoplankton competition.•Fungi and bacteria protect Microcystis aeruginosa from oxidative stress.•Natural H2O2 promote the production of extracellular MC-LR. Hydrogen peroxide (H2O2), which accumulates in water and triggers oxidative stress for aquatic microbes, has been shown to have profound impacts on planktonic microbial community dynamics including cyanobacterial bloom formation. Yet, potential effects of H2O2 on interspecific relationships of phytoplankton-microbe symbiotic interactions remain unclear. Here, we investigated effects of environmentally relevant H2O2 concentrations on interspecific microbial relationships in algae-microbe symbiosis. Microbes play a crucial role in the competition between M. aeruginosa and Chlorella vulgaris at low H2O2 concentrations (∼400 nM), in which fungi and bacteria protect Microcystis aeruginosa from oxidative stress. Moreover, H2O2 stimulated the synthesis and release of extracellular microcystin-LR from Microcystis aeruginosa, while intracellular microcystin-LR concentrations remained at a relatively constant level. In the presence of H2O2, loss of organoheterocyclic compounds, organic acids and ketones contributed to the growth of M. aeruginosa, but the reduction of vitamins inhibited it. Regulation of interspecific relationships by H2O2 is achieved by its action on fungal species and bacterial secretory metabolites. This study explored the response of phytoplankton interspecific relationships in symbiotic phytoplankton-microbe interactions to environmentally relevant H2O2 concentrations stress, providing a theoretical basis for understanding the formation of harmful-algae blooming and impact of photochemical properties of water on aquatic ecological safety and stability. [Display omitted]