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trans -Cleavage of the CRISPR-Cas12a-aptamer system for one-step antigen detection
Rapid detection of prostate-specific antigen (PSA) is pivotal for the early screening of prostate cancer (PCa). Here, we devise a one-step, amplification-free fluorescent detection strategy for PSA, employing the trans -cleavage principle of a CRISPR-Cas12a-aptamer system. This method offers a linea...
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| Published in: | Chemical communications (Cambridge, England) England), 2023-11, Vol.59 (88), p.13151-13154 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c292t-345de325a4ffd8f04d471574ea621b2210629c26eca70677dc8f0d9dcb8eb0a93 |
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| cites | cdi_FETCH-LOGICAL-c292t-345de325a4ffd8f04d471574ea621b2210629c26eca70677dc8f0d9dcb8eb0a93 |
| container_end_page | 13154 |
| container_issue | 88 |
| container_start_page | 13151 |
| container_title | Chemical communications (Cambridge, England) |
| container_volume | 59 |
| creator | Fan, Hongxuan Luo, Shi-hua Zhu, Ying Shi, Jiye Yin, Fangfei Li, Jiang |
| description | Rapid detection of prostate-specific antigen (PSA) is pivotal for the early screening of prostate cancer (PCa). Here, we devise a one-step, amplification-free fluorescent detection strategy for PSA, employing the
trans
-cleavage principle of a CRISPR-Cas12a-aptamer system. This method offers a linear range of 0.31–5 ng mL
−1
and a detection limit of 0.16 ng mL
−1
. The high-confidence quantification of PSA is demonstrated through the analysis of real samples, effectively distinguishing between PCa patients and healthy individuals. |
| doi_str_mv | 10.1039/d3cc04532c |
| format | article |
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trans
-cleavage principle of a CRISPR-Cas12a-aptamer system. This method offers a linear range of 0.31–5 ng mL
−1
and a detection limit of 0.16 ng mL
−1
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trans
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−1
and a detection limit of 0.16 ng mL
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trans
-cleavage principle of a CRISPR-Cas12a-aptamer system. This method offers a linear range of 0.31–5 ng mL
−1
and a detection limit of 0.16 ng mL
−1
. The high-confidence quantification of PSA is demonstrated through the analysis of real samples, effectively distinguishing between PCa patients and healthy individuals.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d3cc04532c</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-2372-6624</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2023-11, Vol.59 (88), p.13151-13154 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2878293932 |
| source | Royal Society of Chemistry |
| subjects | Antigens Cleavage Fluorescence |
| title | trans -Cleavage of the CRISPR-Cas12a-aptamer system for one-step antigen detection |
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