Sterile soil mitigates the intergenerational loss of gut microbial diversity and anxiety-like behavior induced by antibiotics in mice

• Published in: Brain, behavior, and immunity behavior, and immunity, 2024-01, Vol.115, p.179-190
• Main Authors: Li, Na, Xiao, Xiaoao, Zhang, Honglin, Bai, Zhimao, Li, Mengjie, Sun, Jia, Dong, Yangyang, Zhu, Wenyong, Fei, Zhongjie, Sun, Xiao, Xiao, Pengfeng, Gao, Yuanqing, Zhou, Dongrui
• Format: Article
• Language: English
• Subjects: Antibiotic-induced dysbiosis; Anxiety behavior; Hygiene hypothesis; Microglia; Sterile soil
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2023.10.014

•Antibiotic reduces the diversity of gut microbiota in mice over generations.•Intergenerational effect of antibiotics leads to neurodevelopmental deficits.•Sterile soil is a potent therapeutic prebiotic against microbial diversity decline.•Sterile soil intervention ameliorates microgliosis, gut leaking, and behavior changes.•The effect of sterile soil intervention in newborn mice is better than adult mice. The decline in gut microbial diversity in modern humans is closely associated with the rising prevalence of various diseases. It is imperative to investigate the underlying causes of gut microbial loss and restoring methods. Although the impact of non-perinatal antibiotic use on gut microbiota has been recognized, its intergenerational effects remain unexplored. Our previous research has highlighted soil in the farm environment as a key factor for gut microbiome health by restoring gut microbial diversity and balance. In this study, we investigated the intergenerational consequences of antibiotic exposure and the therapeutic potential of sterile soil. We treated C57BL/6 mice with vancomycin and streptomycin for 2 weeks continuously, followed by a 4–8 week withdrawal period before breeding. The process was repeated across 3 generations. Half of the mice in each generation received an oral sterile soil intervention. We assessed gut microbial diversity, anxiety behavior, microglial reactivity, and gut barrier integrity across generations. Antibiotic exposure led to a decrease in gut microbial diversity over generations, along with aggravated anxiety behavior, microgliosis, and altered intestinal tight junction protein expression. Oral sterile soil intervention restored gut microbial diversity in adult mice across generations, concomitantly rescuing abnormalities in behavior, microgliosis, and intestinal barrier integrity. In conclusion, this study simulated an important process of the progressive loss of gut microbiota diversity in modern humans and demonstrated the potential of sterile soil to reverse this process. This study provides a theoretical and experimental basis for research and interventions targeting multiple modern chronic diseases related to intestinal microorganisms.