Evidence for Nitrous Oxide Emissions by Nitrite-Dependent Anaerobic Methane Oxidizing Bacteria

Nitrite-dependent anaerobic methane oxidizing (n-DAMO) bacteria generally convert nitrite to dinitrogen and bypass the nitrous oxide (N2O) formation step. However, N2O is often detected in n-DAMO bacteria dominated cultures and it remains an open question as to the microbial origin of N2O in these e...

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Published in:Environmental science & technology 2023-11, Vol.57 (44), p.16862-16872
Main Authors: Tan, Xin, Lu, Yang, Nie, Wen-Bo, Xie, Guo-Jun, Evans, Paul, Wang, Xiao-Wei, Dang, Cheng-Cheng, Zhao, Zhi-Cheng, Fan, Sheng-Qiang, Ren, Nanqi
Format: Article
Language:English
Subjects:
Bacteria
Biogeochemical Cycling
Bioreactors
Emissions
Enrichment
Enrichment media
Methane
Microorganisms
Nitric oxide
Nitric-oxide reductase
Nitrite reductase
Nitrites
Nitrous oxide
Oxidation
Reductases
Wastewater treatment
Wastewater treatment plants
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Summary:Nitrite-dependent anaerobic methane oxidizing (n-DAMO) bacteria generally convert nitrite to dinitrogen and bypass the nitrous oxide (N2O) formation step. However, N2O is often detected in n-DAMO bacteria dominated cultures and it remains an open question as to the microbial origin of N2O in these enrichments. Using a stable nitrite consuming microbial community enriched for n-DAMO bacteria, we demonstrated that N2O production was coupled to methane oxidation and the higher initial nitrite concentrations led to increased quantities of N2O being formed. Moreover, continuous exposure of the enrichment culture to about 5 mg of N L–1 nitrite resulted in constant N2O being produced (12.5% of nitrite was reduced to N2O). Metatranscriptomic analyses revealed that nitrite reductase (nirS) and nitric oxide reductase (norZ) transcripts from n-DAMO bacteria increased in response to nitrite exposure. No other bacteria significantly expressed nor genes under these conditions, suggesting n-DAMO bacteria are responsible for N2O being produced. In a 35-day bioreactor experiment, N2O produced by the n-DAMO bacteria accumulated when nitrite was in excess; this was found to be up to 3.2% of the nitrogen that resulted from nitrite removal. Together, these results suggested that excess nitrite is an important driver of N2O production by n-DAMO bacteria. To this end, proper monitoring and control of nitrite levels in wastewater treatment plants would be effective strategies for mitigating N2O emissions to the atmosphere.
ISSN:0013-936X
1520-5851
DOI:10.1021/acs.est.3c02805