Multi-regional expression of pancreas-related digestive enzyme genes in the intestinal chamber of the ascidian Ciona intestinalis type A

• Published in: Cell and tissue research 2023-12, Vol.394 (3), p.423-430
• Main Authors: Iguchi, Rin, Usui, Kanae, Nakayama, Satoshi, Sasakura, Yasunori, Sekiguchi, Toshio, Ogasawara, Michio
• Format: Article
• Language: English
• Subjects: adults; Analysis; At-a-glance Article; Biomedical and Life Sciences; Biomedicine; Ciona; Ciona intestinalis; digestion; Digestive enzymes; digestive tract; DNA binding proteins; Enzymes; Epithelial cells; epithelium; Gastrointestinal tract; Gene expression; Genes; Genetic aspects; Genetic transcription; Human Genetics; Hybridization; Intestine; Intestines; juveniles; Molecular Medicine; Pancreas; Proteomics; RNA; sequence analysis; Stomach; Transcription factors; vertebrates
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-023-03839-6

Bilateria share sequential steps in their digestive systems, and digestion occurs in a pre-absorption step within a chamber-like structure. Previous studies on the ascidian Ciona intestinalis type A, an evolutionary research model of vertebrate organs, revealed that Ciona homologs of pancreas-related exocrine digestive enzymes (XDEs) are exclusively expressed in the chamber-like bulging stomach. In the development of the gastrointestinal tract, genes for the pancreas-related transcription factors, namely Ptf1a, Nr5a2, and Pdx, are expressed near the stomach. Recent organ/tissue RNA-seq studies on two Ciona species reported that transcripts of the XDE homologs exist in the intestinal regions, as well as in the stomach. In the present study, we investigated the spatial gene expression of XDE homologs in the gastrointestinal region of the C. intestinalis type A. Whole-mount in situ hybridization using adult and juvenile specimens revealed apparent expression signals of XDE homologs in a small number of gastrointestinal epithelial cells. Furthermore, two pancreas-related transcription factor genes, Nr5a2 and Pdx , exhibited multi-regional expression along the Ciona juvenile intestines. These results imply that ascidians may form multiple digestive regions corresponding to the vertebrate pancreas.